Mouse Anti-DDX27 Recombinant Antibody (2251C2a) (CBMAB-D0545-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DEAD-Box Helicase 27 (DDX27). The antibody can be used for immunoassay techniques, such as WB, ICC, Dot.
See all DDX27 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse

Clone

2251C2a

Antibody Isotype

IgG1

Application

WB, ICC, Dot

Basic Information

Specificity

Human, Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

DEAD (Asp-Glu-Ala-Asp) box polypeptide 27

Introduction

DDX27 is a DEAD box protein involved in the processing of 5.8S and 28S ribosomal RNAs. More specifically, the encoded protein localizes to the nucleolus, where it interacts with the PeBoW complex to ensure proper 3' end formation of 47S rRNA. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Entrez Gene ID

Human	55661
Mouse	228889

UniProt ID

Human	Q96GQ7
Mouse	Q921N6

Alternative Names

DEAD-Box Helicase 27; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 27; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 27; DEAD Box Protein 27; RHLP; Deficiency Of Ribosomal Subunits Protein 1 Homolog; Probable ATP-Dependent RNA Helicase DDX27; RNA Helicase-Like Protein; EC 3.6.4.13;

Function

Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA (PubMed:25825154).

Biological Process

rRNA processing Source: UniProtKB

Cellular Location

Nucleolus; Chromosome. Associates with 60S and 90S pre-ribosomal particles (PubMed:25825154).

References

Li, S., Ma, J., Zheng, A., Song, X., Chen, S., & Jin, F. (2021). DEAD-box helicase 27 enhances stem cell-like properties with poor prognosis in breast cancer. Journal of translational medicine, 19(1), 1-14.

Xiaoqian, W., Bing, Z., Yangwei, L., Yafei, Z., Tingting, Z., Yi, W., ... & Peng, Z. (2021). DEAD-box Helicase 27 Promotes Hepatocellular Carcinoma Progression Through ERK Signaling. Technology in cancer research & treatment, 20, 15330338211055953.

Yang, C., Li, D., Bai, Y., Song, S., Yan, P., Wu, R., ... & Huang, L. (2019). DEAD-box helicase 27 plays a tumor-promoter role by regulating the stem cell-like activity of human colorectal cancer cells. OncoTargets and therapy, 12, 233.

Tang, J., Chen, H., Wong, C. C., Liu, D., Li, T., Wang, X., ... & Yu, J. (2018). DEAD-box helicase 27 promotes colorectal cancer growth and metastasis and predicts poor survival in CRC patients. Oncogene, 37(22), 3006-3021.

Bennett, A. H., O’Donohue, M. F., Gundry, S. R., Chan, A. T., Widrick, J., Draper, I., ... & Gupta, V. A. (2018). RNA helicase, DDX27 regulates skeletal muscle growth and regeneration by modulation of translational processes. PLoS genetics, 14(3), e1007226.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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