Mouse Anti-DDX27 Recombinant Antibody (D0545) (V2LY-0425-LY45)

Name: Mouse Anti-DDX27 Recombinant Antibody (D0545)
SKU: V2LY-0425-LY45
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DDX27 Recombinant Antibody (D0545) (V2LY-0321-LY45-1) in IF

Immunofluorescence on newly isolated (Day 0) and cultured (Day1 and 3) EDL myofibers from wild-type mice (scale bar: 10μm). ...View More

Bennett, A. H., O'Donohue, M. F., Gundry, S. R., Chan, A. T., Widrick, J., Draper, I., ... & Gupta, V. A. (2018). RNA helicase, DDX27 regulates skeletal muscle growth and regeneration by modulation of translational processes. PLoS Genetics, 14(3), e1007226.

Mouse Anti-DDX27 Recombinant Antibody (D0545) (V2LY-0321-LY45-2) in IF

Whole mount immunofluorescence of zebrafish at different time intervals (2 dpf and 4 dpf) with MPC marker (Pax7) and late differentiation marker (Mef2) demonstrating a decrease in of MPC in ddx27 mutant during post-embryonic skeletal muscle growth (4 dpf) (scale bar: 50μm). ...View More

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

D0545

Antibody Isotype

IgG1

Application

WB, IP, FC, IF

Basic Information

Immunogen

N-terminus of human DDX27.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

DEAD (Asp-Glu-Ala-Asp) box polypeptide 27

Entrez Gene ID

Human	55661
Mouse	228889

UniProt ID

Human	Q96GQ7
Mouse	Q921N6

Function

Probable ATP-dependent RNA helicase. Component of the nucleolar ribosomal RNA (rRNA) processing machinery that regulates 3' end formation of ribosomal 47S rRNA (PubMed:25825154).

Biological Process

rRNA processing Source: UniProtKB

Cellular Location

Nucleolus; Chromosome. Associates with 60S and 90S pre-ribosomal particles (PubMed:25825154).

More Infomation

References

Li, S., Ma, J., Zheng, A., Song, X., Chen, S., & Jin, F. (2021). DEAD-box helicase 27 enhances stem cell-like properties with poor prognosis in breast cancer. Journal of translational medicine, 19(1), 1-14.

Xiaoqian, W., Bing, Z., Yangwei, L., Yafei, Z., Tingting, Z., Yi, W., ... & Peng, Z. (2021). DEAD-box Helicase 27 Promotes Hepatocellular Carcinoma Progression Through ERK Signaling. Technology in cancer research & treatment, 20, 15330338211055953.

Yang, C., Li, D., Bai, Y., Song, S., Yan, P., Wu, R., ... & Huang, L. (2019). DEAD-box helicase 27 plays a tumor-promoter role by regulating the stem cell-like activity of human colorectal cancer cells. OncoTargets and therapy, 12, 233.

Tang, J., Chen, H., Wong, C. C., Liu, D., Li, T., Wang, X., ... & Yu, J. (2018). DEAD-box helicase 27 promotes colorectal cancer growth and metastasis and predicts poor survival in CRC patients. Oncogene, 37(22), 3006-3021.

Bennett, A. H., O’Donohue, M. F., Gundry, S. R., Chan, A. T., Widrick, J., Draper, I., ... & Gupta, V. A. (2018). RNA helicase, DDX27 regulates skeletal muscle growth and regeneration by modulation of translational processes. PLoS genetics, 14(3), e1007226.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-DDX27 Recombinant Antibody (2251C2a)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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