Mouse Anti-DDX5 Recombinant Antibody (D0583) (CBMAB-D0583-YC)

Name: Mouse Anti-DDX5 Recombinant Antibody (D0583)
SKU: CBMAB-D0583-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D0583

Application

WB, IF, IHC, FC

Immunogen

E.coli-derived human DDX5 recombinant protein (R85-K328).

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	0.1-0.5 μg/ml
IF(ICC)	2 μg/ml
IHC	0.1-0.5 μg/ml
FC	1-3μg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

Trehalose & PBS

Preservative

None

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

DEAD-Box Helicase 5

Introduction

DDX5 belongs to the DEAD box family of RNA helicases that are involved in a variety of cellular processes as a result of its role as an adaptor molecule, promoting interactions with a large number of other factors. This protein is involved in pathways that include the alteration of RNA structures, plays a role as a coregulator of transcription, a regulator of splicing, and in the processing of small noncoding RNAs. Members of this family contain nine conserved motifs, including the conserved Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and hydrolysis as well as RNA binding and unwinding activities. Dysregulation of this gene may play a role in cancer development.

Entrez Gene ID

Human	1655
Mouse	13207
Rat	287765

UniProt ID

Human	P17844
Mouse	Q61656
Rat	Q6AYI1

Alternative Names

DEAD-Box Helicase 5; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 5 (RNA Helicase, 68kD); DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5; DEAD (Asp-Glu-Ala-Asp) Box Helicase 5; DEAD Box Protein 5; RNA Helicase P68; G17P1; HLR1; Probable ATP-Dependent RNA Helicase DDX5;

Function

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.

Biological Process

Alternative mRNA splicing, via spliceosome Source: UniProtKB
Androgen receptor signaling pathway Source: UniProtKB
BMP signaling pathway Source: BHF-UCL
Epithelial to mesenchymal transition Source: UniProtKB
Intracellular estrogen receptor signaling pathway Source: UniProtKB
Intrinsic apoptotic signaling pathway by p53 class mediator Source: UniProtKB
mRNA splicing, via spliceosome Source: Reactome
mRNA transcription Source: CACAO
Myoblast differentiation Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Nuclear-transcribed mRNA catabolic process Source: UniProtKB
Positive regulation of DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
Positive regulation of production of miRNAs involved in gene silencing by miRNA Source: BHF-UCL
Pri-miRNA transcription by RNA polymerase II Source: UniProtKB
Regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
Regulation of androgen receptor signaling pathway Source: UniProtKB
Regulation of osteoblast differentiation Source: UniProtKB
Regulation of skeletal muscle cell differentiation Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: UniProtKB
Rhythmic process Source: UniProtKB-KW

Cellular Location

Cytoplasm; Nucleus; Nucleolus. During the G0 phase, predominantly located in the nucleus. Cytoplasmic levels increase during the G1/S phase. During the M phase, located at the vicinity of the condensed chromosomes. At G1, localizes in the cytoplasm.

PTM

Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
Sumoylated; sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination.
Polyubiquitinated, leading to proteasomal degradation.
Weakly phosphorylated in the G1/S phase of the cell cycle and much more at G2/M, especially at Thr and Tyr residues.

Xing, Z., Russon, M. P., Utturkar, S. M., & Tran, E. J. (2020). The RNA helicase DDX5 supports mitochondrial function in small cell lung cancer. Journal of Biological Chemistry, 295(27), 8988-8998.

Ma, L., Zhao, X., Wang, S., Zheng, Y., Yang, S., Hou, Y., ... & Dong, L. (2020). Decreased expression of DEAD-Box helicase 5 inhibits esophageal squamous cell carcinomas by regulating endoplasmic reticulum stress and autophagy. Biochemical and Biophysical Research Communications, 533(4), 1449-1456.

Zhao, H., Xie, Z., Tang, G., Wei, S., & Chen, G. (2020). Knockdown of terminal differentiation induced ncRNA (TINCR) suppresses proliferation and invasion in hepatocellular carcinoma by targeting the miR‐218‐5p/DEAD‐box helicase 5 (DDX5) axis. Journal of cellular physiology, 235(10), 6990-7002.

Luo, Q., Que, T., Luo, H., Meng, Y., Chen, X., Huang, H., ... & Huang, G. (2020). Upregulation of DEAD box helicase 5 and 17 are correlated with the progression and poor prognosis in gliomas. Pathology-Research and Practice, 216(3), 152828.

Hashemi, V., Masjedi, A., Hazhir‐karzar, B., Tanomand, A., Shotorbani, S. S., Hojjat‐Farsangi, M., ... & Jadidi‐Niaragh, F. (2019). The role of DEAD‐box RNA helicase p68 (DDX5) in the development and treatment of breast cancer. Journal of cellular physiology, 234(5), 5478-5487.

Fan, Y., Chen, Y., Zhang, J., Yang, F., Hu, Y., Zhang, L., ... & Xu, Q. (2019). Protective role of RNA helicase DEAD-box protein 5 in smooth muscle cell proliferation and vascular remodeling. Circulation Research, 124(10), e84-e100.

Giraud, G., Terrone, S., & Bourgeois, C. F. (2018). Functions of DEAD box RNA helicases DDX5 and DDX17 in chromatin organization and transcriptional regulation. BMB reports, 51(12), 613.

Xing, Z., Wang, S., & Tran, E. J. (2017). Characterization of the mammalian DEAD-box protein DDX5 reveals functional conservation with S. cerevisiae ortholog Dbp2 in transcriptional control and glucose metabolism. RNA, 23(7), 1125-1138.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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