Rabbit Anti-DDX5 Recombinant Antibody (
2C3) (V2LY-0725-LY1082)

Name: Rabbit Anti-DDX5 Recombinant Antibody (2C3)
SKU: V2LY-0725-LY1082
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-DDX5 Recombinant Antibody (2C3) (V2LY-0725-LY1082) in WB

Western Blot
Positive WB detected in: HepG2 whole cell lysate, HT-29 whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, Mouse brain tissue
All lanes: DDX5 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 70, 61 kDa
Observed band size: 70 kDa

Rabbit Anti-DDX5 Recombinant Antibody (2C3) (V2LY-0725-LY1082) in IHC

IHC image of V2LY-0725-LY1082 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-DDX5 Recombinant Antibody (2C3) (V2LY-0725-LY1082) in IF

Immunofluorescence staining of HepG2 Cells with V2LY-0725-LY1082 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Rabbit Anti-DDX5 Recombinant Antibody (2C3) (V2LY-0725-LY1082) in FC

Overlay histogram showing Hela cells stained with V2LY-0725-LY1082 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

2C3

Application

ELISA, WB, IHC, IF, FC

Immunogen

A synthesized peptide derived from human DDX5.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IF	1:20-1:200
FC	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DEAD-Box Helicase 5

Entrez Gene ID

1655

UniProt ID

P17844

Function

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.

Biological Process

Alternative mRNA splicing, via spliceosome Source: UniProtKB
Androgen receptor signaling pathway Source: UniProtKB
BMP signaling pathway Source: BHF-UCL
Epithelial to mesenchymal transition Source: UniProtKB
Intracellular estrogen receptor signaling pathway Source: UniProtKB
Intrinsic apoptotic signaling pathway by p53 class mediator Source: UniProtKB
mRNA splicing, via spliceosome Source: Reactome
mRNA transcription Source: CACAO
Myoblast differentiation Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Nuclear-transcribed mRNA catabolic process Source: UniProtKB
Positive regulation of DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
Positive regulation of production of miRNAs involved in gene silencing by miRNA Source: BHF-UCL
Pri-miRNA transcription by RNA polymerase II Source: UniProtKB
Regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
Regulation of androgen receptor signaling pathway Source: UniProtKB
Regulation of osteoblast differentiation Source: UniProtKB
Regulation of skeletal muscle cell differentiation Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: UniProtKB
Rhythmic process Source: UniProtKB-KW

Cellular Location

Cytoplasm; Nucleus; Nucleolus. During the G0 phase, predominantly located in the nucleus. Cytoplasmic levels increase during the G1/S phase. During the M phase, located at the vicinity of the condensed chromosomes. At G1, localizes in the cytoplasm.

PTM

Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
Sumoylated; sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination.
Polyubiquitinated, leading to proteasomal degradation.
Weakly phosphorylated in the G1/S phase of the cell cycle and much more at G2/M, especially at Thr and Tyr residues.

Xing, Z., Russon, M. P., Utturkar, S. M., & Tran, E. J. (2020). The RNA helicase DDX5 supports mitochondrial function in small cell lung cancer. Journal of Biological Chemistry, 295(27), 8988-8998.

Ma, L., Zhao, X., Wang, S., Zheng, Y., Yang, S., Hou, Y., ... & Dong, L. (2020). Decreased expression of DEAD-Box helicase 5 inhibits esophageal squamous cell carcinomas by regulating endoplasmic reticulum stress and autophagy. Biochemical and Biophysical Research Communications, 533(4), 1449-1456.

Zhao, H., Xie, Z., Tang, G., Wei, S., & Chen, G. (2020). Knockdown of terminal differentiation induced ncRNA (TINCR) suppresses proliferation and invasion in hepatocellular carcinoma by targeting the miR‐218‐5p/DEAD‐box helicase 5 (DDX5) axis. Journal of cellular physiology, 235(10), 6990-7002.

Luo, Q., Que, T., Luo, H., Meng, Y., Chen, X., Huang, H., ... & Huang, G. (2020). Upregulation of DEAD box helicase 5 and 17 are correlated with the progression and poor prognosis in gliomas. Pathology-Research and Practice, 216(3), 152828.

Hashemi, V., Masjedi, A., Hazhir‐karzar, B., Tanomand, A., Shotorbani, S. S., Hojjat‐Farsangi, M., ... & Jadidi‐Niaragh, F. (2019). The role of DEAD‐box RNA helicase p68 (DDX5) in the development and treatment of breast cancer. Journal of cellular physiology, 234(5), 5478-5487.

Fan, Y., Chen, Y., Zhang, J., Yang, F., Hu, Y., Zhang, L., ... & Xu, Q. (2019). Protective role of RNA helicase DEAD-box protein 5 in smooth muscle cell proliferation and vascular remodeling. Circulation Research, 124(10), e84-e100.

Giraud, G., Terrone, S., & Bourgeois, C. F. (2018). Functions of DEAD box RNA helicases DDX5 and DDX17 in chromatin organization and transcriptional regulation. BMB reports, 51(12), 613.

Xing, Z., Wang, S., & Tran, E. J. (2017). Characterization of the mammalian DEAD-box protein DDX5 reveals functional conservation with S. cerevisiae ortholog Dbp2 in transcriptional control and glucose metabolism. RNA, 23(7), 1125-1138.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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