Mouse Anti-DDX60 Recombinant Antibody (1A10) (CBMAB-D0636-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DExD/H-Box Helicase 60 (DDX60). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all DDX60 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1A10

Antibody Isotype

IgG1, κ

Application

ELISA, WB

Basic Information

Immunogen

DDX60 (AAH20601.1, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. The immunogen sequence: MKIMEDFTTF LRIVSKLADM NQEYQLPLSK IKFTGKECED SQLVSHLMSC KEGRVAISPF VCLSGNFDDD LLRLETPNHV TLGTIGVNRS QAPVLLSQKF DNRGRKMSLN AYALDFYKHG SLIGLVQDNR MNEGDAYYLL KDFALTIKSI SVSLRELCEN EDDNVVLAFE QLSTTFWEKL NKV

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

DEAD (Asp-Glu-Ala-Asp) box polypeptide 60

Introduction

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular procsses involving RNA binding and alteration of RNA secondary structure. DDX60 is a DEXD/H box RNA helicase that functions as an antiviral factor and promotes RIG-I-like receptor-mediated signaling.

Entrez Gene ID

55601

UniProt ID

Q8IY21

Alternative Names

DExD/H-Box Helicase 60; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 60; DEAD Box Protein 60; EC 3.6.4.13;

Function

Positively regulates DDX58/RIG-I- and IFIH1/MDA5-dependent type I interferon and interferon inducible gene expression in response to viral infection. Binds ssRNA, dsRNA and dsDNA and can promote the binding of DDX58/RIG-I to dsRNA. Exhibits antiviral activity against hepatitis C virus and vesicular stomatitis virus (VSV).

Biological Process

Defense response to virus Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Positive regulation of MDA-5 signaling pathway Source: UniProtKB
Positive regulation of RIG-I signaling pathway Source: UniProtKB
Response to virus Source: UniProtKB

Cellular Location

Cytoplasm

References

Sadic, M., Schneider, W. M., Katsara, O., Medina, G. N., Mogulothu, A., Yu, Y., ... & Dittmann, M. (2022). Antiviral DExD/H-box helicase 60 selectively inhibits translation from type II internal ribosome entry sites. bioRxiv.

Karasawa, T., Sato, R., Imaizumi, T., Hashimoto, S., Fujita, M., Aizawa, T., ... & Tanaka, H. (2022). Glomerular endothelial expression of type I IFN-stimulated gene, DExD/H-Box helicase 60 via toll-like receptor 3 signaling: possible involvement in the pathogenesis of lupus nephritis. Renal Failure, 44(1), 137-145.

Xin, D., Liu, J., Gu, J., Ji, Y., Jin, J., Sun, L., ... & Tang, Z. (2020). Low expression of DDX60 gene might associate with the radiosensitivity for patients with breast cancer. Journal of oncology, 2020.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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