Mouse Anti-DEDD Recombinant Antibody (1C7) (CBMAB-D0655-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Death Effector Domain Containing (DEDD). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all DEDD antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1C7

Antibody Isotype

IgG1, κ

Application

ELISA, WB

Basic Information

Immunogen

DEDD (NP_127491, 91 a.a. ~ 191 a.a) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

death effector domain containing

Introduction

DEDD is a protein that contains a death effector domain (DED). DED is a protein-protein interaction domain shared by adaptors, regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation, this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF, a basal factor required for RNA polymerase I transcription, in the nucleolus.

Entrez Gene ID

9191

UniProt ID

O75618

Alternative Names

Death Effector Domain Containing; Death Effector Domain-Containing Testicular Molecule; DEDPro1; FLDED-1; DEFT; Death Effector Domain-Containing Protein;

Function

A scaffold protein that directs CASP3 to certain substrates and facilitates their ordered degradation during apoptosis. May also play a role in mediating CASP3 cleavage of KRT18. Regulates degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3. Inhibits DNA transcription in vitro (By similarity).

Biological Process

Extrinsic apoptotic signaling pathway via death domain receptors Source: GO_Central
Regulation of apoptotic process Source: InterPro
Spermatogenesis Source: ProtInc

Cellular Location

Cytoplasm; Nucleolus. Translocated to the nucleus during CD95-mediated apoptosis where it is localized in the nucleoli (By similarity). Following apoptosis induction, the mono and/or diubiquitination form increases and forms filamentous structures that colocalize with KRT8 and KRT18 intermediate filament network in simple epithelial cells.

PTM

Exists predominantly in a mono- or diubiquitinated form.

References

Ni, Y., Schmidt, K. R., Werner, B. A., Koenig, J. K., Guldner, I. H., Schnepp, P. M., ... & Zhang, S. (2019). Death effector domain-containing protein induces vulnerability to cell cycle inhibition in triple-negative breast cancer. Nature communications, 10(1), 1-15.

Su, C., Shi, K., Cheng, X., Han, Y., Li, Y., Yu, D., & Liu, Z. (2018). Long noncoding RNA LINC00472 inhibits proliferation and promotes apoptosis of lung adenocarcinoma cells via regulating miR-24-3p/DEDD. Technology in cancer research & treatment, 17, 1533033818790490.

Wu, D. M., Hong, X. W., Wang, L. L., Cui, X. F., Lu, J., Chen, G. Q., & Zheng, Y. L. (2018). MicroRNA-17 inhibition overcomes chemoresistance and suppresses epithelial-mesenchymal transition through a DEDD-dependent mechanism in gastric cancer. The international journal of biochemistry & cell biology, 102, 59-70.

Yu, G., Jia, Z., & Dou, Z. (2017). miR-24-3p regulates bladder cancer cell proliferation, migration, invasion and autophagy by targeting DEDD. Oncology reports, 37(2), 1123-1131.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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