Mouse Anti-DENR Recombinant Antibody (D0704) (V2LY-0425-LY178)

Name: Mouse Anti-DENR Recombinant Antibody (D0704)
SKU: V2LY-0425-LY178
Rating: 5 (1 reviews)

See all DENR antibodies

Online Inquiry

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

D0704

Antibody Isotype

IgG2a, κ

Application

ELISA, WB, IP

Basic Information

Immunogen

Recombinant human DENR.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:10,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Density Regulated Re-Initiation And Release Factor

Entrez Gene ID

8562

UniProt ID

O43583

Function

May be involved in the translation of target mRNAs by scanning and recognition of the initiation codon. Involved in translation initiation; promotes recruitmnet of aminoacetyled initiator tRNA to P site of 40S ribosomes. Can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits. Plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1.

Biological Process

Formation of translation preinitiation complex Source: UniProtKB
IRES-dependent viral translational initiation Source: UniProtKB
Ribosome disassembly Source: UniProtKB
Translation reinitiation Source: GO_Central

More Infomation

References

Chen, B., Hu, J., Hu, X., Chen, H., Bao, R., Zhou, Y., ... & Li, H. B. (2022). DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion. Nature communications, 13(1), 1-14.

Yu, S. E. (2021). The Role of the MCTS1 and DENR Proteins in Regulating the Mechanisms Associated with Malignant Cell Transformation. Acta Naturae (англоязычная версия), 13(2), 98-105.

Wang, D., Wang, L., Ren, C., Zhang, P., Wang, M., & Zhang, S. (2019). High expression of density‑regulated re‑initiation and release factor drives tumourigenesis and affects clinical outcome. Oncology Letters, 17(1), 141-148.

Ahmed, Y. L., Schleich, S., Bohlen, J., Mandel, N., Simon, B., Sinning, I., & Teleman, A. A. (2018). DENR–MCTS1 heterodimerization and tRNA recruitment are required for translation reinitiation. PLoS biology, 16(6), e2005160.

Lomakin, I. B., Stolboushkina, E. A., Vaidya, A. T., Zhao, C., Garber, M. B., Dmitriev, S. E., & Steitz, T. A. (2017). Crystal structure of the human ribosome in complex with DENR-MCT-1. Cell reports, 20(3), 521-528.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-DENR Recombinant Antibody (D0704)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-DENR Recombinant Antibody (1H3)

Mouse Anti-DENR Recombinant Antibody (CAP621)

Mouse Anti-DENR Recombinant Antibody (D0703)

Mouse Anti-DENR Recombinant Antibody (CAP621)

Mouse Anti-DENR Recombinant Antibody (CBAb081)

Mouse Anti-DENR Recombinant Antibody (1542CT106.51.79)

Recombinant Mouse Anti-DENR Antibody (CBAb081)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: