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Rabbit Anti-DGAT1 Recombinant Antibody (CBYCD-248) (CBMAB-D0760-YC)

Provided herein is a Rabbit monoclonal antibody, which binds to Diacylglycerol O-Acyltransferase 1 (DGAT1). The antibody can be used for immunoassay techniques, such as WB, FC, ICC, IF.
See all DGAT1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
CBYCD-248
Antibody Isotype
IgG
Application
WB, FC, ICC, IF

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Diacylglycerol O-Acyltransferase 1
Introduction
DGAT1 is an multipass transmembrane protein that functions as a key metabolic enzyme. The encoded protein catalyzes the conversion of diacylglycerol and fatty acyl CoA to triacylglycerol. This enzyme can also transfer acyl CoA to retinol. Activity of this protein may be associated with obesity and other metabolic diseases.
Entrez Gene ID
8694
UniProt ID
O75907
Alternative Names
Diacylglycerol O-Acyltransferase 1; Acyl-CoA Retinol O-Fatty-Acyltransferase; Diglyceride Acyltransferase; EC 2.3.1.20; ARAT; DGAT; Acyl Coenzyme A:Cholesterol Acyltransferase Related Gene 1; Diacylglycerol O-Acyltransferase (Mouse) Homolog; Diacylglycerol O-Acyltransferase Homolog; Acyl-CoA:Diacylglycerol Acyltransferase;
Function
Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates (PubMed:16214399, PubMed:18768481, PubMed:28420705, PubMed:9756920, PubMed:32433611, PubMed:32433610).

Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats (PubMed:18768481).

In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage (PubMed:16214399).

Also present in female mammary glands, where it produces fat in the milk (By similarity).

May be involved in VLDL (very low density lipoprotein) assembly (PubMed:18768481).

In contrast to DGAT2 it is not essential for survival (By similarity).

Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders (PubMed:16214399).

Exhibits additional acyltransferase activities, includin acyl CoA:monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester synthases (By similarity).

Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG) (PubMed:28420705).
Biological Process
Acylglycerol acyl-chain remodeling Source: Reactome
Diacylglycerol metabolic process Source: UniProtKB
Fatty acid homeostasis Source: Ensembl
Lipid storage Source: BHF-UCL
Long-chain fatty-acyl-CoA metabolic process Source: BHF-UCL
Monoacylglycerol biosynthetic process Source: UniProtKB
Neutrophil degranulation Source: Reactome
Triglyceride biosynthetic process Source: UniProtKB
Triglyceride metabolic process Source: ProtInc
Very-low-density lipoprotein particle assembly Source: BHF-UCL
Cellular Location
Endoplasmic reticulum membrane
Involvement in disease
Diarrhea 7, protein-losing enteropathy type (DIAR7):
A life-threatening disease characterized by severe, intractable, watery diarrhea.
Topology
Cytoplasmic: 1-83
Helical: 84-118
Lumenal: 119-130
Helical: 131-156
Cytoplasmic: 157-161
Helical: 162-184
Lumenal: 185-191
Helical: 192-223
Cytoplasmic: 224-273
Helical: 274-308
Lumenal: 309-315
Helical: 316-353
Cytoplasmic: 354-399
Helical: 400-420
Lumenal: 421-428
Helical: 429-447
Cytoplasmic: 448-449
Helical: 450-481
Lumenal: 482-488

Yang, W., Wang, S., Loor, J. J., Lopes, M. G., Zhao, Y., Ma, X., ... & Xu, C. (2022). Role of diacylglycerol O-acyltransferase (DGAT) isoforms in bovine hepatic fatty acid metabolism. Journal of Dairy Science, 105(4), 3588-3600.

Xia, L., Wang, Y., Cai, S., & Xu, M. (2021). DGAT1 expression promotes ovarian cancer progression and is associated with poor prognosis. Journal of Immunology Research, 2021.

Torabi, S., Sukumaran, A., Dhaubhadel, S., Johnson, S. E., LaFayette, P., Parrott, W. A., ... & Eskandari, M. (2021). Effects of type I Diacylglycerol O-acyltransferase (DGAT1) genes on soybean (Glycine max L.) seed composition. Scientific reports, 11(1), 1-14.

Wang, L., Qian, H., Nian, Y., Han, Y., Ren, Z., Zhang, H., ... & Zhou, M. (2020). Structure and mechanism of human diacylglycerol O-acyltransferase 1. Nature, 581(7808), 329-332.

Xu, L., Gu, W., Luo, Y., Lou, J., & Chen, J. (2020). DGAT1 mutations leading to delayed chronic diarrhoea: a case report. BMC medical genetics, 21(1), 1-7.

Cheng, X., Geng, F., & Guo, D. (2020). DGAT1 protects tumor from lipotoxicity, emerging as a promising metabolic target for cancer therapy. Molecular & Cellular Oncology, 7(6), 1805257.

Naserkheil, M., Miraie-Ashtiani, S. R., Sadeghi, M., Nejati-Javaremi, A., Park, C. W., Min, K. S., & Lee, D. (2019). Exploring novel single nucleotide polymorphisms and haplotypes of the diacylglycerol O-acyltransferase 1 (DGAT1) gene and their effects on protein structure in Iranian buffalo. Genes & genomics, 41(11), 1265-1271.

Kumar, P., Kumar, A., & Sindhu, J. (2019). In silico design of diacylglycerol acyltransferase-1 (DGAT1) inhibitors based on SMILES descriptors using Monte-Carlo method. SAR and QSAR in Environmental Research, 30(8), 525-541.

Trakovicka, A., Vavrišinova, K., Gabor, M., Miluchova, M., Kasarda, R., & Moravčikova, N. (2019). The impact of diacylglycerol O-acyltransferase 1 gene polymorphism on carcass traits in cattle. Journal of Central European Agriculture, 20(1), 12-18.

Zhang, H., Zhang, Z., Xiong, T., Xiong, X., Wu, X., Guan, C., & Xiao, G. (2018). The CCCH-type transcription factor BnZFP1 is a positive regulator to control oleic acid levels through the expression of diacylglycerol O-acyltransferase 1 gene in Brassica napus. Plant Physiology and Biochemistry, 132, 633-640.

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For research use only. Not intended for any clinical use.

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