Mouse Anti-DGKA Recombinant Antibody (3G7) (V2LY-0425-LY237)

Name: Mouse Anti-DGKA Recombinant Antibody (3G7)
SKU: V2LY-0425-LY237
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3G7

Application

WB, FC

Immunogen

Full length human recombinant protein of human DGKA produced in HEK293T cell.

Host Species

Mouse

Specificity

Human, Rat, Dog

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & Glycerol & PBS

Preservative

Sodium Azide

Concentration

1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Diacylglycerol Kinase Alpha

Entrez Gene ID

Human	1606
Dog	100855691
Rat	140866

UniProt ID

Human	P23743
Dog	E2QW80
Rat	P51556

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:2175712, PubMed:15544348).

Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:2175712, PubMed:15544348).

Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group (PubMed:15544348).

Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol (PubMed:22627129).

Biological Process

Diacylglycerol metabolic process Source: UniProtKB
Glycerolipid metabolic process Source: BHF-UCL
Intracellular signal transduction Source: GO_Central
Lipid phosphorylation Source: UniProtKB
Phosphatidic acid biosynthetic process Source: UniProtKB
Platelet activation Source: Reactome
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: InterPro

Cellular Location

Cytosol

Okada, N., Sugiyama, K., Shichi, S., Shirai, Y., Goto, K., Sakane, F., ... & Taketomi, A. (2022). Combination therapy for hepatocellular carcinoma with diacylglycerol kinase alpha inhibition and anti-programmed cell death-1 ligand blockade. Cancer Immunology, Immunotherapy, 71(4), 889-903.

Li, J., Shen, Y., Chen, Y., Zhang, Z., Ma, S., Wan, Q., ... & Yang, J. (2021). Structure of membrane diacylglycerol kinase in lipid bilayers. Communications biology, 4(1), 1-13.

Liu, C. S., Toth, R., Bakr, A., Goyal, A., Islam, M. S., Breuer, K., ... & Popanda, O. (2021). Epigenetic modulation of radiation-induced diacylglycerol kinase alpha expression prevents pro-fibrotic fibroblast response. Cancers, 13(10), 2455.

Liu, C. S., Schmezer, P., & Popanda, O. (2020). Diacylglycerol kinase alpha in radiation-induced fibrosis: Potential as a predictive marker or therapeutic target. Frontiers in Oncology, 10, 737.

Pan, Y., Deng, W., Xie, J., Zhang, S., Wan, E. C., Li, L., ... & Zhong, X. P. (2020). Graded diacylglycerol kinases α and ζ activities ensure mucosal‐associated invariant T‐cell development in mice. European journal of immunology, 50(2), 192-204.

Chen, J., Zhang, W., Wang, Y., Zhao, D., Wu, M., Fan, J., ... & Zhan, Q. (2019). The diacylglycerol kinase α (DGKα)/Akt/NF-κB feedforward loop promotes esophageal squamous cell carcinoma (ESCC) progression via FAK-dependent and FAK-independent manner. Oncogene, 38(14), 2533-2550.

Olmez, I., Love, S., Xiao, A., Manigat, L., Randolph, P., McKenna, B. D., ... & Purow, B. (2018). Targeting the mesenchymal subtype in glioblastoma and other cancers via inhibition of diacylglycerol kinase alpha. Neuro-oncology, 20(2), 192-202.

Kelly, E., Sharma, D., Wilkinson, C. J., & Williams, R. S. (2018). Diacylglycerol kinase (DGKA) regulates the effect of the epilepsy and bipolar disorder treatment valproic acid in Dictyostelium discoideum. Disease models & mechanisms, 11(9), dmm035600.

Valinciute, G., Weigel, C., Veldwijk, M. R., Oakes, C. C., Herskind, C., Wenz, F., ... & Popanda, O. (2017). BET-bromodomain inhibitors modulate epigenetic patterns at the diacylglycerol kinase alpha enhancer associated with radiation-induced fibrosis. Radiotherapy and Oncology, 125(1), 168-174.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

DGKA Matched Antibody Pair (337) (CAT#: APMAB-337LY)

DGKA Matched Antibody Pair (338) (CAT#: APMAB-338LY)

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DGKA Matched Antibody Pair (337) (CAT#: APMAB-337LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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