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Mouse Anti-DGKH Recombinant Antibody (2G11) (CBMAB-D0790-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Diacylglycerol Kinase Eta (DGKH). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all DGKH antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2G11
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
DGKH (AAH43292, 831 a.a. ~ 930 a.a) partial recombinant protein with GST tag. The immunogen sequence: SGKPVLRTHL YIHHAIDLAT EEVSQMQLCS QAAEELITRI CDAATIHCLL EQELAHAVNA CSHALNKANP RCPESLTRDT ATEIAINVKA LYNETESLLV
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 831-930

Target

Full Name
Diacylglycerol Kinase Eta
Introduction
DGKH belongs to the diacylglycerol kinase (DGK) enzyme family. Members of this family are involved in regulating intracellular concentrations of diacylglycerol and phosphatidic acid. Variation in this gene has been associated with bipolar disorder. Alternatively spliced transcript variants have been identified.
Entrez Gene ID
160851
UniProt ID
Q86XP1
Alternative Names
Diacylglycerol Kinase Eta; Diglyceride Kinase Eta; DAG Kinase Eta; EC 2.7.1.107; DGK-Eta; DGKeta;
Function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:12810723, PubMed:23949095).

Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable) (PubMed:12810723, PubMed:23949095).

Plays a key role in promoting cell growth (PubMed:19710016).

Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF (PubMed:19710016).

Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization (PubMed:19710016).
Biological Process
Diacylglycerol metabolic process Source: UniProtKB
Intracellular signal transduction Source: GO_Central
Lipid phosphorylation Source: UniProtKB
Negative regulation of catalytic activity Source: UniProtKB
Phosphatidic acid biosynthetic process Source: UniProtKB
Phosphatidic acid metabolic process Source: BHF-UCL
Platelet activation Source: Reactome
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: UniProtKB
Cellular Location
Cell membrane; Cytoplasm. Translocated from the cytoplasm to endosomes in response to stress stimuli (PubMed:12810723). Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association (PubMed:12810723). Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS (PubMed:19710016).
PTM
Phosphorylated. Phosphorylation does not inhibit catalytic activity.

Masuda, Y., Murakami, C., Suzuki, R., & Sakane, F. (2022). Diacylglycerol kinase η regulates cell proliferation and its levels are elevated by glucocorticoids in undifferentiated neuroblastoma cells. Biochemical and Biophysical Research Communications, 602, 41-48.

Asami, M., Suzuki, Y., & Sakane, F. (2021). Dopamine and the phosphorylated dopamine transporter are increased in the diacylglycerol kinase η‐knockout mouse brain. FEBS letters, 595(9), 1313-1321.

Suzuki, Y., Asami, M., Takahashi, D., & Sakane, F. (2021). Diacylglycerol kinase η colocalizes and interacts with apoptosis signal-regulating kinase 3 in response to osmotic shock. Biochemistry and biophysics reports, 26, 101006.

Bartsch, V. B., Lord, J. S., Diering, G. H., & Zylka, M. J. (2020). Mania‐and anxiety‐like behavior and impaired maternal care in female diacylglycerol kinase eta and iota double knockout mice. Genes, Brain and Behavior, 19(3), e12570.

Sakai, H., Murakami, C., Usuki, T., Lu, Q., Matsumoto, K. I., Urano, T., & Sakane, F. (2020). Diacylglycerol kinase η regulates C2C12 myoblast proliferation through the mTOR signaling pathway. Biochimie, 177, 13-24.

Komenoi, S., Suzuki, Y., Asami, M., Murakami, C., Hoshino, F., Chiba, S., ... & Sakane, F. (2019). Microarray analysis of gene expression in the diacylglycerol kinase η knockout mouse brain. Biochemistry and biophysics reports, 19, 100660.

Palladino, V. S., Subrata, N. O. C., Geburtig-Chiocchetti, A., McNeill, R., Hoffmann, P., Reif, A., & Kittel-Schneider, S. (2018). Generation of human induced pluripotent stem cell lines (hiPSC) from one bipolar disorder patient carrier of a DGKH risk haplotype and one non-risk-variant-carrier bipolar disorder patient. Stem Cell Research, 32, 104-109.

Weißflog, L., Becker, N., Bossert, N., Freudenberg, F., Kittel-Schneider, S., & Reif, A. (2017). Expressional profile of the diacylglycerol kinase eta gene DGKH. European Archives of Psychiatry and Clinical Neuroscience, 267(5), 445-454.

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For research use only. Not intended for any clinical use.

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