Mouse Anti-DGKH Recombinant Antibody (2G11) (V2LY-0425-LY249)

Name: Mouse Anti-DGKH Recombinant Antibody (2G11)
SKU: V2LY-0425-LY249
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2G11

Application

ELISA, WB

Immunogen

DGKH (aa831-930) partial recombinant protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Diacylglycerol Kinase Eta

Entrez Gene ID

160851

UniProt ID

Q86XP1

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:12810723, PubMed:23949095).

Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable) (PubMed:12810723, PubMed:23949095).

Plays a key role in promoting cell growth (PubMed:19710016).

Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF (PubMed:19710016).

Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization (PubMed:19710016).

Biological Process

Diacylglycerol metabolic process Source: UniProtKB
Intracellular signal transduction Source: GO_Central
Lipid phosphorylation Source: UniProtKB
Negative regulation of catalytic activity Source: UniProtKB
Phosphatidic acid biosynthetic process Source: UniProtKB
Phosphatidic acid metabolic process Source: BHF-UCL
Platelet activation Source: Reactome
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: UniProtKB

Cellular Location

Cell membrane; Cytoplasm. Translocated from the cytoplasm to endosomes in response to stress stimuli (PubMed:12810723). Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association (PubMed:12810723). Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS (PubMed:19710016).

PTM

Phosphorylated. Phosphorylation does not inhibit catalytic activity.

Masuda, Y., Murakami, C., Suzuki, R., & Sakane, F. (2022). Diacylglycerol kinase η regulates cell proliferation and its levels are elevated by glucocorticoids in undifferentiated neuroblastoma cells. Biochemical and Biophysical Research Communications, 602, 41-48.

Asami, M., Suzuki, Y., & Sakane, F. (2021). Dopamine and the phosphorylated dopamine transporter are increased in the diacylglycerol kinase η‐knockout mouse brain. FEBS letters, 595(9), 1313-1321.

Suzuki, Y., Asami, M., Takahashi, D., & Sakane, F. (2021). Diacylglycerol kinase η colocalizes and interacts with apoptosis signal-regulating kinase 3 in response to osmotic shock. Biochemistry and biophysics reports, 26, 101006.

Bartsch, V. B., Lord, J. S., Diering, G. H., & Zylka, M. J. (2020). Mania‐and anxiety‐like behavior and impaired maternal care in female diacylglycerol kinase eta and iota double knockout mice. Genes, Brain and Behavior, 19(3), e12570.

Sakai, H., Murakami, C., Usuki, T., Lu, Q., Matsumoto, K. I., Urano, T., & Sakane, F. (2020). Diacylglycerol kinase η regulates C2C12 myoblast proliferation through the mTOR signaling pathway. Biochimie, 177, 13-24.

Komenoi, S., Suzuki, Y., Asami, M., Murakami, C., Hoshino, F., Chiba, S., ... & Sakane, F. (2019). Microarray analysis of gene expression in the diacylglycerol kinase η knockout mouse brain. Biochemistry and biophysics reports, 19, 100660.

Palladino, V. S., Subrata, N. O. C., Geburtig-Chiocchetti, A., McNeill, R., Hoffmann, P., Reif, A., & Kittel-Schneider, S. (2018). Generation of human induced pluripotent stem cell lines (hiPSC) from one bipolar disorder patient carrier of a DGKH risk haplotype and one non-risk-variant-carrier bipolar disorder patient. Stem Cell Research, 32, 104-109.

Weißflog, L., Becker, N., Bossert, N., Freudenberg, F., Kittel-Schneider, S., & Reif, A. (2017). Expressional profile of the diacylglycerol kinase eta gene DGKH. European Archives of Psychiatry and Clinical Neuroscience, 267(5), 445-454.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Mouse Anti-DGKH Recombinant Antibody (2G11) (CAT#: CBMAB-D0790-YC)

Related Products

Mouse Anti-DGKH Recombinant Antibody (2G11) (CAT#: CBMAB-D0790-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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