Mouse Anti-DHPS Recombinant Antibody (2C9) (CBMAB-D0840-YC)

Name: Mouse Anti-DHPS Recombinant Antibody (2C9)
SKU: CBMAB-D0840-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2C9

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 37-71 near the N-terminus of human DHS.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Dog, Pig

Antibody Isotype

IgG3

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Deoxyhypusine Synthase

Introduction

DHPS is a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue.

Entrez Gene ID

1725

UniProt ID

P49366

Alternative Names

Deoxyhypusine Synthase; Migration-Inducing Gene 13; EC 2.5.1.46; DHS; DS; EC 2.5.1; MIG13;

Function

Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a critical lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue (PubMed:30661771).

This is the first step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.

Biological Process

Peptidyl-lysine modification to peptidyl-hypusine Source: UniProtKB
Positive regulation of cell population proliferation Source: ProtInc
Spermidine metabolic process Source: UniProtKB
Translation Source: ProtInc

Cellular Location

Cytosol; Cytoplasm

Involvement in disease

Neurodevelopmental disorder with seizures and speech and walking impairment (NEDSSWI):
An autosomal recessive disorder characterized by global developmental delay with intellectual disability and poor speech acquisition, and walking difficulties due to hypotonia, hypertonia, spasticity, or poor coordination. Additional features include seizures, mild dysmorphic features, and variable short stature.

Kar, R. K., Hanner, A. S., Starost, M. F., Springer, D., Mastracci, T. L., Mirmira, R. G., & Park, M. H. (2021). Neuron-specific ablation of eIF5A or deoxyhypusine synthase leads to impairments in growth, viability, neurodevelopment, and cognitive functions in mice. Journal of Biological Chemistry, 297(5).

Padgett, L. R., Robertson, M. A., Anderson‐Baucum, E. K., Connors, C. T., Wu, W., Mirmira, R. G., & Mastracci, T. L. (2021). Deoxyhypusine synthase, an essential enzyme for hypusine biosynthesis, is required for proper exocrine pancreas development. The FASEB Journal, 35(5), e21473.

Anderson-Baucum, E., Piñeros, A. R., Kulkarni, A., Webb-Robertson, B. J., Maier, B., Anderson, R. M., ... & Mirmira, R. G. (2021). Deoxyhypusine synthase promotes a pro-inflammatory macrophage phenotype. Cell Metabolism, 33(9), 1883-1893.

Kar, R. K., Hanner, A. S., Starost, M. F., Springer, D., Mastracci, T. L., Mirmira, R. G., & Park, M. H. (2021). Neuron specific ablation of eIF5A or deoxyhypusine synthase leads to impairment in development and cognitive functions in mice. bioRxiv.

Liu, K. L., Li, X. Y., Wang, D. P., Xue, W. H., Qian, X. H., Li, Y. H., ... & Meng, F. H. (2021). Novel Allosteric Inhibitors of Deoxyhypusine Synthase against Malignant Melanoma: Design, Synthesis, and Biological Evaluation. Journal of Medicinal Chemistry, 64(18), 13356-13372.

Tanaka, Y., Kurasawa, O., Yokota, A., Klein, M. G., Ono, K., Saito, B., ... & Imamura, S. (2020). Discovery of novel allosteric inhibitors of deoxyhypusine synthase. Journal of Medicinal Chemistry, 63(6), 3215-3226.

Tanaka, Y., Kurasawa, O., Yokota, A., Klein, M. G., Saito, B., Matsumoto, S., ... & Imamura, S. (2020). New series of potent allosteric inhibitors of deoxyhypusine synthase. ACS Medicinal Chemistry Letters, 11(8), 1645-1652.

Ganapathi, M., Padgett, L. R., Yamada, K., Devinsky, O., Willaert, R., Person, R., ... & Chung, W. K. (2019). Recessive rare variants in deoxyhypusine synthase, an enzyme involved in the synthesis of hypusine, are associated with a neurodevelopmental disorder. The American Journal of Human Genetics, 104(2), 287-298.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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