Mouse Anti-DHRS2 Recombinant Antibody (1F10) (CBMAB-D0842-YC)

Name: Mouse Anti-DHRS2 Recombinant Antibody (1F10)
SKU: CBMAB-D0842-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1F10

Application

ELISA, WB

Immunogen

DHRS2 (NP_878912.1, 229 a.a. ~ 300 a.a) partial recombinant protein with GST tag. The immunogen sequence: GFMGMSLSGR TSRNIISCRG LGSQRTVQES CPSCALQMPA TSTGRTLRWQ ATPLGSERSG GGCVAVVPGP GA

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 229-300

More Infomation

Target

Full Name

Dehydrogenase/Reductase 2

Introduction

DHRS2 belongs to the short-chain dehydrogenases/reductases (SDR) family, which has over 46,000 members. Members of this family are enzymes that metabolize many different compounds, such as steroid hormones, prostaglandins, retinoids, lipids and xenobiotics. Alternative promoter use and alternative splicing results in multiple transcript variants.

Entrez Gene ID

10202

UniProt ID

Q13268

Alternative Names

Dehydrogenase/Reductase 2; Short Chain Dehydrogenase/Reductase Family 25C Member 1; Dehydrogenase/Reductase (SDR Family) Member 2; Dicarbonyl Reductase HEP27; Protein D; SDR25C1; Dehydrogenase/Reductase SDR Family Member 2, Mitochondrial;

Function

Displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates. No reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. Attenuates MDM2-mediated p53/TP53 degradation, leading to p53/TP53 stabilization and increased transcription activity, resulting in the accumulation of MDM2 and CDKN1A/p21.

Biological Process

C21-steroid hormone metabolic process Source: UniProtKB
Cellular response to oxidative stress Source: UniProtKB
Electron transport chain Source: UniProtKB
Myeloid dendritic cell differentiation Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Response to toxic substance Source: UniProtKB

Cellular Location

Mitochondrion matrix; Nucleus. A minor fraction of the protein is translocated from the mitochondria to the nucleus, after cleavage of the targeting signal.

Li, Z., Liu, H., Bode, A., & Luo, X. (2021). Emerging roles of dehydrogenase/reductase member 2 (DHRS2) in the pathology of disease. European Journal of Pharmacology, 898, 173972.

Yaylaz, B. S., Ekmekci, S. S., Çınar, S., Deniz, G., & Abacı, N. (2021). DHRS2 is a potential marker of breast cancer metastasis. Gene Reports, 25, 101302.

Yamashita, T. (2020). HSF1 Regulates Cellular Senescence: Role of the DHRS2-MDM2-p53 Pathway. In Heat Shock Proteins in Human Diseases (pp. 237-248). Springer, Cham.

Nettersheim, D., Berger, D., Jostes, S., Skowron, M., & Schorle, H. (2019). Deciphering the molecular effects of romidepsin on germ cell tumours: DHRS 2 is involved in cell cycle arrest but not apoptosis or induction of romidepsin effectors. Journal of Cellular and Molecular Medicine, 23(1), 670-679.

Li, J. M., Jiang, G. M., Zhao, L., Yang, F., Yuan, W. Q., Wang, H., & Luo, Y. Q. (2019). Dehydrogenase/reductase SDR family member 2 silencing sensitizes an oxaliplatin‑resistant cell line to oxaliplatin by inhibiting excision repair cross‑complementing group 1 protein expression. Oncology Reports, 42(5), 1725-1734.

Luo, X., Li, N., Zhao, X., Liao, C., Ye, R., Cheng, C., ... & Cao, Y. (2019). DHRS2 mediates cell growth inhibition induced by Trichothecin in nasopharyngeal carcinoma. Journal of Experimental & Clinical Cancer Research, 38(1), 1-14.

Zhou, Y., Wang, L., Ban, X., Zeng, T., Zhu, Y., Li, M., ... & Li, Y. (2018). DHRS2 inhibits cell growth and motility in esophageal squamous cell carcinoma. Oncogene, 37(8), 1086-1094.

Han, Y., Song, C., Wang, J., Tang, H., Peng, Z., & Lu, S. (2018). HOXA13 contributes to gastric carcinogenesis through DHRS2 interacting with MDM2 and confers 5‐FU resistance by a p53‐dependent pathway. Molecular carcinogenesis, 57(6), 722-734.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Mouse Anti-DHRS2 Recombinant Antibody (1F10) (CAT#: V2LY-0425-LY283)

Related Products

Mouse Anti-DHRS2 Recombinant Antibody (1F10) (CAT#: V2LY-0425-LY283)

Isotype control

For research use only. Not intended for any clinical use.

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