Mouse Anti-DIMT1 Recombinant Antibody (B-11) (CBMAB-D0932-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DIM1 Dimethyladenosine Transferase 1 Homolog (DIMT1). The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all DIMT1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

B-11

Antibody Isotype

IgG

Application

WB, IP, IF, ELISA

Basic Information

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

DIM1 Dimethyladenosine Transferase 1 Homolog

Introduction

DIMT1 is a methyltransferase that is responsible for dimethylation of adjacent adenosines near the 18S rRNA decoding site. The protein is essential for ribosome biogenesis, although its catalytic activity is not involved in the process. The yeast ortholog of this protein functions in the cytoplasm while this protein functions in the nucleus.

Entrez Gene ID

Human	27292
Mouse	66254
Rat	294718

UniProt ID

Human	Q9UNQ2
Mouse	Q9D0D4
Rat	G3V7R8

Alternative Names

DIM1 Dimethyladenosine Transferase 1 Homolog; Probable 18S RRNA (Adenine(1779)-N(6)/Adenine(1780)-N(6))-Dimethyltransferase; Probable S-Adenosylmethionine-6-N,N-Adenosyl(RRNA) Dimethyltransferase; DIM1 Dimethyladenosine Transferase 1-Like; Probable 18S RRNA Dimethylase; DIMT1L; 18S RRNA (Adenine(1779)-N(6)/Adenine(1780)-N(6))-Dimethyltransferase; S-Adenosylmethionine-6-N,N-Adenosyl(RRNA) Dimethyltransferase; DIM1 Dimethyladenosine Transferase 1 Homolog (S. Cerevisiae);

Function

Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle (PubMed:25851604).

Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity (PubMed:25851604).

Biological Process

Positive regulation of rRNA processing Source: UniProtKB
rRNA methylation Source: UniProtKB

Cellular Location

Nucleoplasm; Nucleolus

References

Verma, G., Bowen, A., Gheibi, S., Hamilton, A., Muthukumar, S., Cataldo, L. R., ... & Mulder, H. (2022). Ribosomal biogenesis regulator DIMT1 controls β-cell protein synthesis, mitochondrial function, and insulin secretion. Journal of Biological Chemistry, 298(3).

Shen, H., Gonskikh, Y., Stoute, J., & Liu, K. F. (2021). Human DIMT1 generates N26, 6A-dimethylation–containing small RNAs. Journal of Biological Chemistry, 297(4).

Shen, H., Stoute, J., & Liu, K. F. (2020). Structural and catalytic roles of the human 18S rRNA methyltransferases DIMT1 in ribosome assembly and translation. Journal of Biological Chemistry, 295(34), 12058-12070.

Verma, G., Bowen, A., Hamilton, A., Esguerra, J., Karagiannopoulos, A., Cataldo, L. R., ... & Mulder, H. (2020). DIMT1, a regulator of ribosomal biogenesis, controls β-cell protein synthesis, mitochondrial function and insulin secretion. bioRxiv.

Tran, H. Q., Van Vo, T., & Nguyen, M. N. (2020). High expression of DIMT1 correlates with poor prognosis and high risk of bone metastasis in breast cancer patients. Science & Technology Development Journal-Health Sciences, 1(2), 52-63.

Ikeda, S., Kitadate, A., Abe, F., Saitoh, H., Michishita, Y., Hatano, Y., ... & Tagawa, H. (2017). Hypoxia‐inducible micro RNA‐210 regulates the DIMT 1‐IRF 4 oncogenic axis in multiple myeloma. Cancer science, 108(4), 641-652.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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