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Mouse Anti-DIS3 Recombinant Antibody (2C7) (CBMAB-D0939-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DIS3 Homolog, Exosome Endoribonuclease And 3'-5' Exoribonuclease (DIS3). The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all DIS3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2C7
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
KIAA1008 (NP_055768, 861 a.a. ~ 956 a.a) partial recombinant protein with GST tag. The immunogen sequence: VVLIPKYGLE GTVFFEEKDK PNPQLIYDDE IPSLKIEDTV FHVFDKVKVK IMLDSSNLQH QKIRMSLVEP QIPGISIPTD TSNMDLNGPK KKKMKL
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 861-956

Target

Full Name
DIS3
Introduction
DIS3 (DIS3 Homolog, Exosome Endoribonuclease And 3'-5' Exoribonuclease) is a protein coding gene. Diseases associated with DIS3 include Combat Disorder and Acute Stress Disorder. Among its related pathways are CDK-mediated phosphorylation and removal of Cdc6 and Gene Expression. It has proved that the protein has RNA binding and endonuclease activity. And a paralog is DIS3L.
Entrez Gene ID
22894
UniProt ID
Q9Y2L1
Alternative Names
DIS3 Homolog, Exosome Endoribonuclease And 3'-5' Exoribonuclease; DIS3 Homolog, Exosome Endoribonuclease And 3-5 Exoribonuclease; KIAA1008; Ribosomal RNA-Processing Protein 44; Exosome Component 11; RRP44; DIS3 Exosome Endoribonuclease And 3-5 Exoribonuclease; DIS3 Mitotic Control Homolog (S. Cerevisiae); Mitotic Control Protein Dis3 Homolog; Exosome Complex Exonuclease RRP44;
Function
Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.
Biological Process
CUT catabolic process Source: UniProtKB
Exonucleolytic catabolism of deadenylated mRNA Source: Reactome
rRNA catabolic process Source: UniProtKB
rRNA processing Source: UniProtKB
Cellular Location
Cytoplasm; Nucleolus; Nucleoplasm; Nucleus. Predominantly located in the nucleus (PubMed:20531386). According to PubMed:12429849, found in the nucleolus (PubMed:12429849). According to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3 (PubMed:20531386).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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