Mouse Anti-DLST Recombinant Antibody (4D7) (CBMAB-D1107-YC)

Name: Mouse Anti-DLST Recombinant Antibody (4D7)
SKU: CBMAB-D1107-YC
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Rat

Clone

4D7

Antibody Isotype

IgG2b, κ

Application

ELISA, WB

Basic Information

Immunogen

DLST (aa1-99) partial recombinant protein.

Host Species

Mouse

Specificity

Human, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Dihydrolipoamide S-Succinyltransferase

Introduction

DLST is a mitochondrial protein that belongs to the 2-oxoacid dehydrogenase family. This protein is one of the three components (the E2 component) of the 2-oxoglutarate dehydrogenase complex that catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). Alternatively spliced transcript variants have been found for this gene.

Entrez Gene ID

1743

UniProt ID

P36957

Alternative Names

Dihydrolipoamide S-Succinyltransferase; Dihydrolipoamide S-Succinyltransferase (E2 Component Of 2-Oxo-Glutarate Complex); EC 2.3.1.61; OGDC-E2; DLTS; E2K;

Function

Dihydrolipoamide succinyltransferase (E2) component of the 2-oxoglutarate dehydrogenase complex. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (PubMed:29211711, PubMed:30929736).

A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (PubMed:29211711).

Biological Process

2-oxoglutarate metabolic process Source: UniProtKB
Generation of precursor metabolites and energy Source: ProtInc
Histone succinylation Source: UniProtKB
L-lysine catabolic process to acetyl-CoA via saccharopine Source: UniProtKB-UniPathway
Succinyl-CoA metabolic process Source: UniProtKB
Tricarboxylic acid cycle Source: UniProtKB

Cellular Location

Mitochondrion matrix; Nucleus. Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2-oxoglutarate dehydrogenase complex is required for histone succinylation.

Involvement in disease

Paragangliomas 7 (PGL7):
An autosomal dominant tumor predisposition syndrome characterized by adult-onset development of paragangliomas, neural crest tumors usually derived from the chromoreceptor tissue of a paraganglion. PGL7 tumors are generally benign, tend to be abdominal, and often secrete normetanephrine.

More Infomation

References

Wei, C., Huang, J., Wang, Y., Chen, Y., Luo, X., Wang, S., ... & Chen, J. (2021). Discovery of Novel Dihydrolipoamide S-Succinyltransferase Inhibitors Based on Fragment Virtual Screening. International Journal of Molecular Sciences, 22(23), 12953.

Shen, N., Korm, S., Karantanos, T., Li, D., Zhang, X., Ritou, E., ... & Feng, H. (2021). DLST-dependence dictates metabolic heterogeneity in TCA-cycle usage among triple-negative breast cancer. Communications biology, 4(1), 1-11.

Anderson, N. M., Qin, X., Finan, J. M., Lam, A., Athoe, J., Missiaen, R., ... & Feng, H. (2021). Metabolic enzyme DLST promotes tumor aggression and reveals a vulnerability to OXPHOS inhibition in high-risk neuroblastoma. Cancer research, 81(17), 4417-4430.

Buffet, A., Zhang, J., Rebel, H., Corssmit, E. P., Jansen, J. C., Hensen, E. F., ... & Bayley, J. P. (2021). Germline DLST variants promote epigenetic modifications in pheochromocytoma-paraganglioma. The Journal of Clinical Endocrinology & Metabolism, 106(2), 459-471.

Chen, B., Long, Q., Zhao, Y., Wu, Y., Ge, S., Wang, P., ... & Yang, S. (2019). Sulfone-based probes unraveled dihydrolipoamide S-Succinyltransferase as an unprecedented target in phytopathogens. Journal of agricultural and food chemistry, 67(25), 6962-6969.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Rabbit Anti-DLST Recombinant Antibody (D1109)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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