Mouse Anti-DLST Recombinant Antibody (4D7) (CBMAB-D1107-YC)

Name: Mouse Anti-DLST Recombinant Antibody (4D7)
SKU: CBMAB-D1107-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4D7

Application

ELISA, WB

Immunogen

DLST (aa1-99) partial recombinant protein.

Host Species

Mouse

Specificity

Human, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Dihydrolipoamide S-Succinyltransferase

Introduction

DLST is a mitochondrial protein that belongs to the 2-oxoacid dehydrogenase family. This protein is one of the three components (the E2 component) of the 2-oxoglutarate dehydrogenase complex that catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). Alternatively spliced transcript variants have been found for this gene.

Entrez Gene ID

1743

UniProt ID

P36957

Alternative Names

Dihydrolipoamide S-Succinyltransferase; Dihydrolipoamide S-Succinyltransferase (E2 Component Of 2-Oxo-Glutarate Complex); EC 2.3.1.61; OGDC-E2; DLTS; E2K;

Function

Dihydrolipoamide succinyltransferase (E2) component of the 2-oxoglutarate dehydrogenase complex. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (PubMed:29211711, PubMed:30929736).

A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (PubMed:29211711).

Biological Process

2-oxoglutarate metabolic process Source: UniProtKB
Generation of precursor metabolites and energy Source: ProtInc
Histone succinylation Source: UniProtKB
L-lysine catabolic process to acetyl-CoA via saccharopine Source: UniProtKB-UniPathway
Succinyl-CoA metabolic process Source: UniProtKB
Tricarboxylic acid cycle Source: UniProtKB

Cellular Location

Mitochondrion matrix; Nucleus. Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2-oxoglutarate dehydrogenase complex is required for histone succinylation.

Involvement in disease

Paragangliomas 7 (PGL7):
An autosomal dominant tumor predisposition syndrome characterized by adult-onset development of paragangliomas, neural crest tumors usually derived from the chromoreceptor tissue of a paraganglion. PGL7 tumors are generally benign, tend to be abdominal, and often secrete normetanephrine.

Wei, C., Huang, J., Wang, Y., Chen, Y., Luo, X., Wang, S., ... & Chen, J. (2021). Discovery of Novel Dihydrolipoamide S-Succinyltransferase Inhibitors Based on Fragment Virtual Screening. International Journal of Molecular Sciences, 22(23), 12953.

Shen, N., Korm, S., Karantanos, T., Li, D., Zhang, X., Ritou, E., ... & Feng, H. (2021). DLST-dependence dictates metabolic heterogeneity in TCA-cycle usage among triple-negative breast cancer. Communications biology, 4(1), 1-11.

Anderson, N. M., Qin, X., Finan, J. M., Lam, A., Athoe, J., Missiaen, R., ... & Feng, H. (2021). Metabolic enzyme DLST promotes tumor aggression and reveals a vulnerability to OXPHOS inhibition in high-risk neuroblastoma. Cancer research, 81(17), 4417-4430.

Buffet, A., Zhang, J., Rebel, H., Corssmit, E. P., Jansen, J. C., Hensen, E. F., ... & Bayley, J. P. (2021). Germline DLST variants promote epigenetic modifications in pheochromocytoma-paraganglioma. The Journal of Clinical Endocrinology & Metabolism, 106(2), 459-471.

Chen, B., Long, Q., Zhao, Y., Wu, Y., Ge, S., Wang, P., ... & Yang, S. (2019). Sulfone-based probes unraveled dihydrolipoamide S-Succinyltransferase as an unprecedented target in phytopathogens. Journal of agricultural and food chemistry, 67(25), 6962-6969.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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