Mouse Anti-DLX5 Recombinant Antibody (9G371) (V2LY-0425-LY533)

Name: Mouse Anti-DLX5 Recombinant Antibody (9G371)
SKU: V2LY-0425-LY533
Rating: 5 (1 reviews)

See all DLX5 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

9G371

Application

ELISA, IF

Immunogen

DLX5 (aa1-110) partial recombinant protein.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IF(ICC)	10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

distal-less homeobox 5

Entrez Gene ID

Human	1749
Mouse	13395
Rat	25431

UniProt ID

Human	P56178
Mouse	P70396
Rat	P50575

Function

Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding.

Biological Process

Anatomical structure formation involved in morphogenesis Source: GO_Central
BMP signaling pathway Source: BHF-UCL
Cell differentiation Source: GO_Central
Cell population proliferation Source: UniProtKB
Cellular response to BMP stimulus Source: BHF-UCL
Embryonic limb morphogenesis Source: Ensembl
Endochondral ossification Source: UniProtKB
Epithelial cell differentiation Source: Ensembl
Face morphogenesis Source: Ensembl
Inner ear morphogenesis Source: Ensembl
Interneuron axon guidance Source: Ensembl
Nervous system development Source: ProtInc
Olfactory bulb interneuron differentiation Source: Ensembl
Olfactory pit development Source: Ensembl
Osteoblast differentiation Source: UniProtKB
Positive regulation of canonical Wnt signaling pathway Source: Ensembl
Positive regulation of epithelial cell proliferation Source: Ensembl
Positive regulation of gene expression Source: Ensembl
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus Source: BHF-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Roof of mouth development Source: Ensembl
Skeletal system development Source: ProtInc

Cellular Location

Nucleus

Involvement in disease

Split-hand/foot malformation 1 with sensorineural hearing loss, autosomal recessive (SHFM1D):
A disease characterized by the association of split-hand/foot malformation with deafness. Split-hand/foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. Some patients have been found to have mental retardation, ectodermal and craniofacial findings, and orofacial clefting.

PTM

Phosphorylated. Phosphorylation of Ser-34 and Ser-217 by MAPK14 enhances its transcriptional activity. Phosphorylation by CaMK2 increases its protein stability (By similarity).

Mullen, R. D., Bellessort, B., Levi, G., & Behringer, R. D. (2022). Distal-less homeobox genes Dlx5/6 regulate Mulllerian duct regression. bioRxiv.

Kim, C. Y., Cho, D. H., Chung, D. J., Lee, S. H., Han, Y., & Lee, K. Y. (2021). Dlx5 Represses the Transcriptional Activity of PPARγ. Biological and Pharmaceutical Bulletin, 44(9), 1303-1308.

Zhang, G., Zhou, Y., Su, M., Yang, X., & Zeng, B. (2020). Inhibition of microRNA‐27b‐3p relieves osteoarthritis pain via regulation of KDM4B‐dependent DLX5. Biofactors, 46(5), 788-802.

Sun, S., Yang, F., Zhu, Y., & Zhang, S. (2020). KDM4A promotes the growth of non-small cell lung cancer by mediating the expression of Myc via DLX5 through the Wnt/β-catenin signaling pathway. Life Sciences, 262, 118508.

Yang, H., Fan, J., Cao, Y., Gao, R., & Fan, Z. (2019). Distal-less homeobox 5 promotes the osteo-/dentinogenic differentiation potential of stem cells from apical papilla by activating histone demethylase KDM4B through a positive feedback mechanism. Experimental Cell Research, 374(1), 221-230.

Yang, D., Okamura, H., & Qiu, L. (2018). Upregulated osterix expression elicited by Runx2 and Dlx5 is required for the accelerated osteoblast differentiation in PP2A Cα‐knockdown cells. Cell Biology International, 42(4), 403-410.

Lee, K. M., Park, K. H., Hwang, J. S., Lee, M., Yoon, D. S., Ryu, H. A., ... & Lee, J. W. (2018). Inhibition of STAT5A promotes osteogenesis by DLX5 regulation. Cell death & disease, 9(11), 1-13.

Lee, H. L., Qadir, A. S., Park, H. J., Chung, E., Lee, Y. S., Woo, K. M., ... & Baek, J. H. (2018). cAMP/protein kinase A signaling inhibits Dlx5 expression via activation of CREB and subsequent C/EBPβ induction in 3T3-L1 preadipocytes. International Journal of Molecular Sciences, 19(10), 3161.

Heo, J. S., Lee, S. G., & Kim, H. O. (2017). Distal-less homeobox 5 is a master regulator of the osteogenesis of human mesenchymal stem cells. International journal of molecular medicine, 40(5), 1486-1494.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-DLX5 Recombinant Antibody (9G371)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-DLX5 Recombinant Antibody (CBYCD-313) (CAT#: V2LY-0425-LY532)

Rabbit Anti-DLX5 Recombinant Antibody (1031) (CAT#: V2LY-0425-LY528)

Mouse Anti-DLX5 Recombinant Antibody (3B11) (CAT#: CBMAB-D1154-YC)

Mouse Anti-DLX5 Recombinant Antibody (4B7) (CAT#: CBMAB-D1146-YC)

Mouse Anti-DLX5 Recombinant Antibody (1B7) (CAT#: V2LY-0425-LY529)

Mouse Anti-DLX5 Recombinant Antibody (3F5) (CAT#: V2LY-0425-LY530)

Mouse Anti-DLX5 Recombinant Antibody (2B6) (CAT#: CBMAB-D1152-YC)

Mouse Anti-DLX5 Recombinant Antibody (4C6) (CAT#: CBMAB-D1155-YC)

Mouse Anti-DLX5 Recombinant Antibody (4F5) (CAT#: V2LY-0425-LY535)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry