Mouse Anti-DMAP1 Recombinant Antibody (5H4) (CBMAB-A2289-LY)

Name: Mouse Anti-DMAP1 Recombinant Antibody (5H4)
SKU: CBMAB-A2289-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

5H4

Application

WB, ELISA

Immunogen

DMAP1 (NP_061973, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DNA Methyltransferase 1 Associated Protein 1

Introduction

This gene encodes a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Entrez Gene ID

55929

UniProt ID

Q9NPF5

Alternative Names

DKFZp686L09142; DNMAP1; DNMTAP1; EAF2; FLJ11543; KIAA1425; SWC4

Function

Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity.

Biological Process

DNA methylation Source: UniProtKB
DNA repair Source: InterPro
Histone exchange Source: GO_Central
Histone H2A acetylation Source: UniProtKB
Histone H4 acetylation Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of protein import into nucleus Source: UniProtKB
Regulation of growth Source: UniProtKB-KW
Response to ethanol Source: Ensembl

Cellular Location

Cytoplasm; Nucleus. Targeted to replication foci throughout S phase by DNMT1.

Li, Y. Y., Tian, C. B., Wu, Y. X., Niu, T. D., Wang, H., Fan, W. H., & Liu, H. (2021). Enhanced expression of DNA methyltransferase 1-associated protein1 gene thermotolerance in a high-temperature acclimated predatory mite Neoseiulus barkeri. BioControl, 66(6), 779-788.

Gegner, J., Gegner, T., Vogel, H., & Vilcinskas, A. (2020). Silencing of the DNA methyltransferase 1 associated protein 1 (DMAP1) gene in the invasive ladybird Harmonia axyridis implies a role of the DNA methyltransferase 1‐DMAP1 complex in female fecundity. Insect Molecular Biology, 29(2), 148-159.

Kurz, L., Miklyaeva, A., Skowron, M. A., Overbeck, N., Poschmann, G., Becker, T., ... & Nettersheim, D. (2020). ARID1A regulates transcription and the epigenetic landscape via POLE and DMAP1 while ARID1A deficiency or pharmacological inhibition sensitizes germ cell tumor cells to ATR inhibition. Cancers, 12(4), 905.

Yu, J. S., Palano, G., Lim, C., Moggio, A., Drowley, L., Plowright, A. T., ... & Yusa, K. (2019). CRISPR-knockout screen identifies Dmap1 as a regulator of chemically induced reprogramming and differentiation of cardiac progenitors. Stem Cells, 37(7), 958-972.

Howard, A. E. (2017). Characterization of DNA Methyltransferase 1-Associated Protein from Phytophthora sojae (Doctoral dissertation, Bowling Green State University).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-DMAP1 Recombinant Antibody (5H4) (CAT#: V2LY-0425-LY542)

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Isotype control

For research use only. Not intended for any clinical use.

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