Mouse Anti-DMP1 Recombinant Antibody (1D4) (CBMAB-D1198-YC)

Name: Mouse Anti-DMP1 Recombinant Antibody (1D4)
SKU: CBMAB-D1198-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1D4

Application

ELISA, IF, WB

Immunogen

DMP1 (NP_004398.1, 221 a.a. ~ 330 a.a) partial recombinant protein with GST tag. The immunogen sequence: ESIRSERGNS RMNSAGMKSK ESGENSEQAN TQDSGGSQLL EHPSRKIFRK SRISEEDDRS ELDDNNTMEE VKSDSTENSN SRDTGLSQPR RDSKGDSQED SKENLSQEES

Specificity

Human

Antibody Isotype

IgG2b, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

dentin matrix acidic phosphoprotein 1

Introduction

DMP1, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. DMP1 contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in DMP1 are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia.

Entrez Gene ID

1758

UniProt ID

Q13316

Alternative Names

Dentin Matrix Acidic Phosphoprotein 1; Dentin Matrix Protein 1; DMP-1; Dentin Matrix Acidic Phosphoprotein; ARHP; ARHR;

Function

May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.

Biological Process

Biomineral tissue development Source: UniProtKB-KW
Extracellular matrix organization Source: Ensembl
Ossification Source: ProtInc
Positive regulation of cell-substrate adhesion Source: Ensembl
Regulation of enamel mineralization Source: Ensembl

Cellular Location

Cytoplasm; Extracellular matrix; Nucleus. In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralized matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus.

Involvement in disease

Hypophosphatemic rickets, autosomal recessive, 1 (ARHR1):
A hereditary form of hypophosphatemic rickets, a disorder of proximal renal tubule function that causes phosphate loss, hypophosphatemia and skeletal deformities, including rickets and osteomalacia unresponsive to vitamin D. Symptoms are bone pain, fractures and growth abnormalities.

PTM

Phosphorylated in the cytosol and extracellular matrix and unphosphorylated in the nucleus. Phosphorylation is necessary for nucleocytoplasmic transport and may be catalyzed by a nuclear isoform of CK2 and can be augmented by calcium. Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif.

Martin, A., & Kentrup, D. (2021). The Role of DMP1 in CKD-MBD. Current osteoporosis reports, 19(5), 500-509.

Yamada, M., Nagayama, M., Miyamoto, Y., Kawano, S., Takitani, Y., Tanaka, M., ... & Yoshida, T. (2021). Mineral Trioxide Aggregate (MTA) Upregulates the Expression of DMP1 in Direct Pulp Capping in the Rat Molar. Materials, 14(16), 4640.

Whyte, M. P., Amalnath, S. D., McAlister, W. H., McKee, M. D., Veis, D. J., Huskey, M., ... & Mumm, S. (2020). Hypophosphatemic osteosclerosis, hyperostosis, and enthesopathy associated with novel homozygous mutations of DMP1 encoding dentin matrix protein 1 and SPP1 encoding osteopontin: The first digenic SIBLING protein osteopathy?. Bone, 132, 115190.

Oya, K., Ishida, K., Nishida, T., Sato, S., Kishino, M., Hirose, K., ... & Toyosawa, S. (2017). Immunohistochemical analysis of dentin matrix protein 1 (Dmp1) phosphorylation by Fam20C in bone: implications for the induction of biomineralization. Histochemistry and cell biology, 147(3), 341-351.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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