Human Recombinant DMP1 protein, His Tag (V2LY-0526-LY3600)

Name: Human Recombinant DMP1 protein, His Tag
SKU: V2LY-0526-LY3600
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant DMP1 protein, His Tag consist of Amino Acid: 1-513 and predicts a molecular mass of 55.4 kDa.

Molecule Mass

55.4 kDa

Sequence

Amino Acid: 1-513

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile MOPS, NaCl, Chaps

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

dentin matrix acidic phosphoprotein 1

Function

May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.

Biological Process

Biomineral tissue development Source: UniProtKB-KW
Extracellular matrix organization Source: Ensembl
Ossification Source: ProtInc
Positive regulation of cell-substrate adhesion Source: Ensembl
Regulation of enamel mineralization Source: Ensembl

Cellular Location

Cytoplasm; Extracellular matrix; Nucleus. In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralized matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus.

Involvement in disease

Hypophosphatemic rickets, autosomal recessive, 1 (ARHR1):
A hereditary form of hypophosphatemic rickets, a disorder of proximal renal tubule function that causes phosphate loss, hypophosphatemia and skeletal deformities, including rickets and osteomalacia unresponsive to vitamin D. Symptoms are bone pain, fractures and growth abnormalities.

PTM

Phosphorylated in the cytosol and extracellular matrix and unphosphorylated in the nucleus. Phosphorylation is necessary for nucleocytoplasmic transport and may be catalyzed by a nuclear isoform of CK2 and can be augmented by calcium. Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif.

Martin, A., & Kentrup, D. (2021). The Role of DMP1 in CKD-MBD. Current osteoporosis reports, 19(5), 500-509.

Yamada, M., Nagayama, M., Miyamoto, Y., Kawano, S., Takitani, Y., Tanaka, M., ... & Yoshida, T. (2021). Mineral Trioxide Aggregate (MTA) Upregulates the Expression of DMP1 in Direct Pulp Capping in the Rat Molar. Materials, 14(16), 4640.

Whyte, M. P., Amalnath, S. D., McAlister, W. H., McKee, M. D., Veis, D. J., Huskey, M., ... & Mumm, S. (2020). Hypophosphatemic osteosclerosis, hyperostosis, and enthesopathy associated with novel homozygous mutations of DMP1 encoding dentin matrix protein 1 and SPP1 encoding osteopontin: The first digenic SIBLING protein osteopathy?. Bone, 132, 115190.

Oya, K., Ishida, K., Nishida, T., Sato, S., Kishino, M., Hirose, K., ... & Toyosawa, S. (2017). Immunohistochemical analysis of dentin matrix protein 1 (Dmp1) phosphorylation by Fam20C in bone: implications for the induction of biomineralization. Histochemistry and cell biology, 147(3), 341-351.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rabbit Anti-DMP1 Polyclonal Antibody (PTM-CBPAB-0579YC) (CAT#: PTM-CBPAB-0579YC)

Related Products

Mouse Anti-DMP1 Recombinant Antibody (D1198) (CAT#: V2LY-0425-LY575)

Rabbit Anti-DMP1 Recombinant Antibody (CBPAB-0579YC) (CAT#: V2LY-0425-LY576)

Mouse Anti-DMP1 Recombinant Antibody (1D4) (CAT#: CBMAB-D1198-YC)

Rabbit Anti-DMP1 Polyclonal Antibody (PTM-CBPAB-0579YC) (CAT#: PTM-CBPAB-0579YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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