Mouse Anti-DNAI2 Recombinant Antibody (CBXC-0640) (V2LY-0425-LY609)

Name: Mouse Anti-DNAI2 Recombinant Antibody (CBXC-0640)
SKU: V2LY-0425-LY609
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DNAI2 Recombinant Antibody (CBXC-0640) (V2LY-0425-LY609-1) in IF

A-D Immunofluorescence (IF) analysis of tracheal multi-ciliate epithelium showed a loss of outer dynein arm (ODA) subunits in Lrrc6 KO mice. Red, (A, B) DNAH5 and (C, D) DNAI2; green, acetylated α tubulin; blue, DAPI. ...View More

Kim, D. Y., Sub, Y. J., Kim, H. Y., Cho, K. J., Choi, W. I., Choi, Y. J., ... & Gee, H. Y. (2023). LRRC6 regulates biogenesis of motile cilia by aiding FOXJ1 translocation into the nucleus. Cell Communication and Signaling, 21(1), 142.

Mouse Anti-DNAI2 Recombinant Antibody (CBXC-0640) (V2LY-0425-LY609-2) in WB

Overall protein expression level of ODAs was lower in the testicular extracts from Lrrc6 KO than that in WT mice. ...View More

Mouse Anti-DNAI2 Recombinant Antibody (CBXC-0640) (V2LY-0425-LY609-3) in IF

Immunofluorescence analysis of acetylated α-tubulin (Ac-α-tub, green) and DNAI2 (red) expression in mTEC cultures at ALI day 14. DNAI2 was significantly decreased and did not colocalize with acetylated α-tubulin in Zmynd10−/− mTECs, suggesting that motile cilia lacked ODAs. Scale bars, 10 μm. ...View More

Cho, K. J., Noh, S. H., Han, S. M., Choi, W. I., Kim, H. Y., Yu, S., ... & Gee, H. Y. (2018). ZMYND10 stabilizes intermediate chain proteins in the cytoplasmic pre-assembly of dynein arms. PLoS Genetics, 14(3), e1007316.

Mouse Anti-DNAI2 Recombinant Antibody (CBXC-0640) (V2LY-0425-LY609-4) in WB

Representative immunoblot analyses of DNAH7, DNAI2, DNAI1, IQUB, LRRC6, C21ORF59, and ZMYND10 in the testis extracts from Zmynd10+/+ and Zmynd10−/− mice. ...View More

Mouse Anti-DNAI2 Recombinant Antibody (CBXC-0640) (V2LY-0425-LY609-5) in IF

(A-C) Immunostaining of control human nasal brush epithelia reveals endogenous HEATR2 (red: Novus (A), Proteintech (C)) is highly enriched in the cytoplasm of developing MMC when components of outer dynein arms (B: DNAH5, red; C: DNAI2: green) as well as inner dynein arms (A-C: DNALI1, purple) are predominantly cytoplasmic. Arrowheads highlight fully mature MMCs where these components are exclusively axonemal and with relatively lower levels of HEATR2. Arrows highlight immature MMCs for comparison. Nuclei are stained with DAPI (blue). ...View More

Diggle, C. P., Moore, D. J., Mali, G., Zur Lage, P., Ait-Lounis, A., Schmidts, M., ... & Mill, P. (2014). HEATR2 plays a conserved role in assembly of the ciliary motile apparatus. PLoS genetics, 10(9), e1004577.

Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-0640

Application

ELISA, IF, WB

Immunogen

Full length recombinant DNAI2.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IF(ICC)	10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Dynein Axonemal Intermediate Chain 2

Entrez Gene ID

64446

UniProt ID

Q9GZS0

Function

Part of the dynein complex of respiratory cilia.

Biological Process

Cilium assembly Source: MGI
Cilium movement Source: SYSCILIA_CCNET
Determination of left/right symmetry Source: SYSCILIA_CCNET
Microtubule-based movement Source: GO_Central
Outer dynein arm assembly Source: SYSCILIA_CCNET

Cellular Location

Cilium axoneme; Dynein axonemal particle

Involvement in disease

Ciliary dyskinesia, primary, 9 (CILD9):
A disorder characterized by abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit randomization of left-right body asymmetry and situs inversus, due to dysfunction of monocilia at the embryonic node. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.

Lv, Y., Liu, Y., Wang, Y., Kong, F., Pang, Q., & Hu, G. (2021). CCDC114, DNAI2 and TOP2A involves in the effects of tibolone treatment on postmenopausal endometrium. BMC Women's Health, 21(1), 1-10.

Rocca, M. S., Piatti, G., Michelucci, A., Guazzo, R., Bertini, V., Vinanzi, C., ... & Foresta, C. (2020). A novel genetic variant in DNAI2 detected by custom gene panel in a newborn with Primary Ciliary Dyskinesia: case report. BMC medical genetics, 21(1), 1-6.

Rocca, M., Vinanzi, C., Michelucci, A., Caligo, M., Valetto, A., De Santi, M., ... & Foresta, C. (2020, June). A new genetic variants in DNAI2 detected by target sequencing in a newborn with primary ciliary dyskinesia. In The European Human Genetic virtual Conference.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-DNAI2 Recombinant Antibody (CBXC-0640) (CAT#: CBMAB-C5725-CQ)

Antibody Pairs

DNAI2 Matched Antibody Pair (343) (CAT#: APMAB-343LY)

DNAI2 Matched Antibody Pair (344) (CAT#: APMAB-344LY)

Related Products

Mouse Anti-DNAI2 Recombinant Antibody (CBXC-0640) (CAT#: CBMAB-C5725-CQ)

DNAI2 Matched Antibody Pair (344) (CAT#: APMAB-344LY)

Mouse Anti-DNAI2 (AA 1-594) Recombinant Antibody (CBFYC-2588) (CAT#: CBMAB-C2662-FY)

DNAI2 Matched Antibody Pair (343) (CAT#: APMAB-343LY)

Mouse Anti-DNAI2 Recombinant Antibody (CBFYC-2588) (CAT#: V2LY-0425-LY608)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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