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Mouse Anti-DNAJA3 Recombinant Antibody (CBYJT-1185) (CBMAB-T0199-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to DNAJA3 (DnaJ Heat Shock Protein Family (Hsp40) Member A3). The antibody can be used for immunoassay techniques, such as IP, WB.
See all DNAJA3 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYJT-1185
Antibody Isotype
IgG1, κ
Application
IP, WB

Basic Information

Immunogen
Recombinant hTid-1 protein. Cellular Localization: Mitochondria. MW of Antigen: 40 and 43kD
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
10 mM PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DnaJ Heat Shock Protein Family (Hsp40) Member A3
Introduction
DNAJA3 is a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. DNAJA3 is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. DNAJA3 also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein.
Entrez Gene ID
Human9093
Mouse83945
Rat360481
UniProt ID
HumanQ96EY1
MouseQ99M87
RatQ2TVU3
Alternative Names
DnaJ Heat Shock Protein Family (Hsp40) Member A3; Hepatocellular Carcinoma-Associated Antigen 57; Tumorous Imaginal Discs Protein Tid56 Homolog; DnaJ (Hsp40) Homolog, Subfamily A, Member 3; DnaJ Protein Tid-1; HTID-1; HCA57; TID1; DnaJ Homolog Subfamily A Member 3, Mitochondrial
Function
Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.
Biological Process
Activation-induced cell death of T cells Source: Ensembl
Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Cell aging Source: Ensembl
Mitochondrial DNA replication Source: Ensembl
Mitochondrion organization Source: GO_Central
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Negative regulation of interferon-gamma-mediated signaling pathway Source: UniProtKB
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
Negative regulation of programmed cell death Source: Ensembl
Negative regulation of protein kinase activity Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Neuromuscular junction development Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of protein ubiquitination Source: UniProtKB
Positive regulation of T cell proliferation Source: Ensembl
Protein folding Source: UniProtKB
Protein stabilization Source: UniProtKB
Regulation of apoptotic process Source: GO_Central
Response to heat Source: InterPro
Response to interferon-gamma Source: UniProtKB
Skeletal muscle acetylcholine-gated channel clustering Source: UniProtKB
Small GTPase mediated signal transduction Source: Ensembl
T cell differentiation in thymus Source: Ensembl
Cellular Location
Mitochondrion matrix; Cytosol; Postsynaptic cell membrane. Recruited to the postsynaptic cell membrane of the neuromuscular junction through interaction with MUSK.
PTM
Tyrosine phosphorylated.

Maletzko, A., Key, J., Wittig, I., Gispert, S., Koepf, G., Canet-Pons, J., ... & Auburger, G. (2021). Increased presence of nuclear DNAJA3 and upregulation of cytosolic STAT1 and of nucleic acid sensors trigger innate immunity in the ClpP-null mouse. neurogenetics, 22(4), 297-312.

Chen, K. Y., Huang, Y. H., Teo, W. H., Chang, C. W., Chen, Y. S., Yeh, Y. C., ... & Lo, J. F. (2021). Loss of Tid1/DNAJA3 co-chaperone promotes progression and recurrence of hepatocellular carcinoma after surgical resection: a novel model to stratify risk of recurrence. Cancers, 13(1), 138.

Wang, S. F., Huang, K. H., Tseng, W. C., Lo, J. F., Li, A. F. Y., Fang, W. L., ... & Lee, H. C. (2020). DNAJA3/Tid1 Is Required for Mitochondrial DNA Maintenance and Regulates Migration and Invasion of Human Gastric Cancer Cells. Cancers, 12(11), 3463.

Teo, W. H., Fann, Y. N., & Lo, J. F. (2020). DNAJA3, a Co-chaperone in Development and Tumorigenesis. In Heat Shock Proteins in Inflammatory Diseases (pp. 307-323). Springer, Cham.

Zhang, W., Yang, F., Zhu, Z., Yang, Y., Wang, Z., Cao, W., ... & Zheng, H. (2019). Cellular DNAJA3, a novel VP1-interacting protein, inhibits foot-and-mouth disease virus replication by inducing lysosomal degradation of VP1 and attenuating its antagonistic role in the beta interferon signaling pathway. Journal of virology, 93(13), e00588-19.

Kumada, K., Fuse, N., Tamura, T., Okamori, C., & Kurata, S. (2019). HSP70/DNAJA3 chaperone/cochaperone regulates NF-κB activity in immune responses. Biochemical and biophysical research communications, 513(4), 947-951.

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For research use only. Not intended for any clinical use.

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