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Mouse Anti-DNAJA3 Recombinant Antibody (CBYJT-1186) (V2LY-0425-LY618)


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Published Data
Mouse Anti-DNAJA3 Recombinant Antibody (CBYJT-1186) (V2LY-0425-LY618-1) in WB
Mouse Anti-DNAJA3 Recombinant Antibody (CBYJT-1186) (V2LY-0425-LY618-1) in WB
Western blot analysis of mitochondrial chaperons, co-chaperons, and protease (left), and band intensity quantification (right). ...View More
Ishikawa, K., Kobayashi, K., Yamada, A., Umehara, M., Oka, T., & Nakada, K. (2019). Concentration of mitochondrial DNA mutations by cytoplasmic transfer from platelets to cultured mouse cells. Plos one, 14(3), e0213283.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYJT-1186
Antibody Isotype
IgG1
Application
IP, WB

Basic Information

Immunogen
Recombinant human Tid-1 protein.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:1,000
IP1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
BSA & Glycerol & HEPES
Preservative
Sodium Azide
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DnaJ Heat Shock Protein Family (Hsp40) Member A3
Entrez Gene ID
Human9093
Mouse83945
Rat360481
UniProt ID
HumanQ96EY1
MouseQ99M87
RatQ2TVU3
Function
Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.
Biological Process
Activation-induced cell death of T cells Source: Ensembl
Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Cell aging Source: Ensembl
Mitochondrial DNA replication Source: Ensembl
Mitochondrion organization Source: GO_Central
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Negative regulation of interferon-gamma-mediated signaling pathway Source: UniProtKB
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
Negative regulation of programmed cell death Source: Ensembl
Negative regulation of protein kinase activity Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Neuromuscular junction development Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of protein ubiquitination Source: UniProtKB
Positive regulation of T cell proliferation Source: Ensembl
Protein folding Source: UniProtKB
Protein stabilization Source: UniProtKB
Regulation of apoptotic process Source: GO_Central
Response to heat Source: InterPro
Response to interferon-gamma Source: UniProtKB
Skeletal muscle acetylcholine-gated channel clustering Source: UniProtKB
Small GTPase mediated signal transduction Source: Ensembl
T cell differentiation in thymus Source: Ensembl
Cellular Location
Mitochondrion matrix; Cytosol; Postsynaptic cell membrane. Recruited to the postsynaptic cell membrane of the neuromuscular junction through interaction with MUSK.
PTM
Tyrosine phosphorylated.
More Infomation

Maletzko, A., Key, J., Wittig, I., Gispert, S., Koepf, G., Canet-Pons, J., ... & Auburger, G. (2021). Increased presence of nuclear DNAJA3 and upregulation of cytosolic STAT1 and of nucleic acid sensors trigger innate immunity in the ClpP-null mouse. neurogenetics, 22(4), 297-312.

Chen, K. Y., Huang, Y. H., Teo, W. H., Chang, C. W., Chen, Y. S., Yeh, Y. C., ... & Lo, J. F. (2021). Loss of Tid1/DNAJA3 co-chaperone promotes progression and recurrence of hepatocellular carcinoma after surgical resection: a novel model to stratify risk of recurrence. Cancers, 13(1), 138.

Wang, S. F., Huang, K. H., Tseng, W. C., Lo, J. F., Li, A. F. Y., Fang, W. L., ... & Lee, H. C. (2020). DNAJA3/Tid1 Is Required for Mitochondrial DNA Maintenance and Regulates Migration and Invasion of Human Gastric Cancer Cells. Cancers, 12(11), 3463.

Teo, W. H., Fann, Y. N., & Lo, J. F. (2020). DNAJA3, a Co-chaperone in Development and Tumorigenesis. In Heat Shock Proteins in Inflammatory Diseases (pp. 307-323). Springer, Cham.

Zhang, W., Yang, F., Zhu, Z., Yang, Y., Wang, Z., Cao, W., ... & Zheng, H. (2019). Cellular DNAJA3, a novel VP1-interacting protein, inhibits foot-and-mouth disease virus replication by inducing lysosomal degradation of VP1 and attenuating its antagonistic role in the beta interferon signaling pathway. Journal of virology, 93(13), e00588-19.

Kumada, K., Fuse, N., Tamura, T., Okamori, C., & Kurata, S. (2019). HSP70/DNAJA3 chaperone/cochaperone regulates NF-κB activity in immune responses. Biochemical and biophysical research communications, 513(4), 947-951.

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For research use only. Not intended for any clinical use.

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