Mouse Anti-DNAJB2 Recombinant Antibody (4D12) (CBMAB-D1288-YC)

Name: Mouse Anti-DNAJB2 Recombinant Antibody (4D12)
SKU: CBMAB-D1288-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4D12

Application

Immunogen

Human DNAJB2.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Glycerol & PBS

Preservative

Sodium Azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

DnaJ Heat Shock Protein Family (Hsp40) Member B2

Introduction

DNAJB2 is almost exclusively expressed in the brain, mainly in the neuronal layers. It encodes a protein that shows sequence similarity to bacterial DnaJ protein and the yeast homologs. In bacteria, this protein is implicated in protein folding and protein complex dissociation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Entrez Gene ID

3300

UniProt ID

P25686

Alternative Names

DnaJ Heat Shock Protein Family (Hsp40) Member B2; DnaJ (Hsp40) Homolog, Subfamily B, Member 2; Heat Shock 40 KDa Protein 3; Heat Shock Protein J1; HSJ-1; HSPF3; HSJ1;

Function

Functions as a co-chaperone, regulating the substrate binding and activating the ATPase activity of chaperones of the HSP70/heat shock protein 70 family (PubMed:7957263, PubMed:22219199).

In parallel, also contributes to the ubiquitin-dependent proteasomal degradation of misfolded proteins (PubMed:15936278, PubMed:21625540).

Thereby, may regulate the aggregation and promote the functional recovery of misfolded proteins like HTT, MC4R, PRKN, RHO and SOD1 and be crucial for many biological processes (PubMed:12754272, PubMed:20889486, PubMed:21719532, PubMed:22396390, PubMed:24023695).

Isoform 1 which is localized to the endoplasmic reticulum membranes may specifically function in ER-associated protein degradation of misfolded proteins (PubMed:15936278).

Biological Process

Chaperone-mediated protein folding Source: UniProtKB
Negative regulation of cell growth Source: UniProtKB
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of inclusion body assembly Source: BHF-UCL
Negative regulation of protein binding Source: UniProtKB
Negative regulation of protein deubiquitination Source: BHF-UCL
Neuron cellular homeostasis Source: ARUK-UCL
Positive regulation of ATPase activity Source: UniProtKB
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: BHF-UCL
Positive regulation of protein ubiquitination Source: BHF-UCL
Proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
Protein refolding Source: UniProtKB
Regulation of chaperone-mediated protein folding Source: UniProtKB
Regulation of protein localization Source: ParkinsonsUK-UCL
Regulation of protein ubiquitination Source: UniProtKB
Response to unfolded protein Source: ProtInc
Ubiquitin-dependent ERAD pathway Source: BHF-UCL

Cellular Location

Isoform 1: Endoplasmic reticulum membrane
Isoform 2: Cytoplasm; Nucleus

Involvement in disease

Distal spinal muscular atrophy, autosomal recessive, 5 (DSMA5):
An autosomal recessive neurologic disorder characterized by young adult onset of slowly progressive distal muscle weakness and atrophy resulting in gait impairment and loss of reflexes due to impaired function of motor nerves. Sensation and cognition are not impaired.

PTM

Ubiquitinated by STUB1; does not lead to proteasomal degradation.

Saveri, P., Magri, S., Maderna, E., Balistreri, F., Lombardi, R., Ciano, C., ... & Pisciotta, C. (2022). DNAJB2‐related Charcot‐Marie‐Tooth disease type 2: Pathomechanism insights and phenotypic spectrum widening. European Journal of Neurology.

Sarparanta, J., Jonson, P., Vihola, A., Luque, H., Reimann, J., Kornblum, C., & Udd, B. (2020). HEREDITARY NEUROPATHIES & ALS: P. 114 DNAJB2 mutation causes dominant neuromyopathy. Neuromuscular Disorders, 30, S79-S80.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

DNAJB2 Matched Antibody Pair (347) (CAT#: APMAB-347LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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