Mouse Anti-DNAJB9 Recombinant Antibody (CBFYE-0352) (CBMAB-E0646-FY)

Name: Mouse Anti-DNAJB9 Recombinant Antibody (CBFYE-0352)
SKU: CBMAB-E0646-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0352

Application

ELISA, IF

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

DnaJ (Hsp40) homolog, subfamily B, member 9

Introduction

This gene is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. This gene is a member of the type 2 subgroup of DnaJ proteins. The encoded protein is localized to the endoplasmic reticulum. This protein is induced by endoplasmic reticulum stress and plays a role in protecting stressed cells from apoptosis.

Entrez Gene ID

4189

UniProt ID

Q9UBS3

Alternative Names

DnaJ Heat Shock Protein Family (Hsp40) Member B9; Microvascular Endothelial Differentiation Gene 1 Protein; Endoplasmic Reticulum DNA J Domain-Containing Protein 4; DnaJ (Hsp40) Homolog, Subfamily B, Member 9; ER-Resident Protein ERdj4; MDG-1; ERdj4

Function

Co-chaperone for Hsp70 protein HSPA5/BiP that acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (By similarity).

J domain-containing co-chaperones stimulate the ATPase activity of Hsp70 proteins and are required for efficient substrate recognition by Hsp70 proteins (PubMed:18400946).

In the unstressed endoplasmic reticulum, interacts with the luminal region of ERN1/IRE1 and selectively recruits HSPA5/BiP: HSPA5/BiP disrupts the dimerization of the active ERN1/IRE1 luminal region, thereby inactivating ERN1/IRE1 (By similarity).

Also involved in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Required for survival of B-cell progenitors and normal antibody production (By similarity).

Biological Process

B cell differentiation Source: UniProtKB
Negative regulation of IRE1-mediated unfolded protein response Source: UniProtKB
Positive regulation of immunoglobulin production Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB
Response to unfolded protein Source: UniProtKB-KW
Ubiquitin-dependent ERAD pathway Source: UniProtKB

Cellular Location

Endoplasmic reticulum lumen

Kim, H. Y., Kim, Y. M., & Hong, S. (2021). DNAJB9 suppresses the metastasis of triple-negative breast cancer by promoting FBXO45-mediated degradation of ZEB1. Cell Death & Disease, 12(5), 1-12.

Said, S. M., Rocha, A. B., Royal, V., Valeri, A. M., Larsen, C. P., Theis, J. D., ... & Nasr, S. H. (2021). Immunoglobulin-negative DNAJB9-associated fibrillary glomerulonephritis: a report of 9 cases. American Journal of Kidney Diseases, 77(3), 454-458.

Wang, D., Zeng, T., Lin, Z., Yan, L., Wang, F., Tang, L., ... & Yang, M. (2020). Long non-coding RNA SNHG5 regulates chemotherapy resistance through the miR-32/DNAJB9 axis in acute myeloid leukemia. Biomedicine & Pharmacotherapy, 123, 109802.

Sun, F., Liao, Y., Qu, X., Xiao, X., Hou, S., Chen, Z., ... & Fu, S. (2020). Hepatic DNAJB9 drives anabolic biasing to reduce steatosis and obesity. Cell Reports, 30(6), 1835-1847.

Lee, H. J., Jung, Y. J., Lee, S., Kim, J. I., & Han, J. A. (2020). DNAJB9 Inhibits p53-dependent oncogene-induced senescence and induces cell transformation. Molecules and cells, 43(4), 397.

Said, S. M., Leung, N., Alexander, M. P., Cornell, L. D., Fidler, M. E., Grande, J. P., ... & Nasr, S. H. (2020). DNAJB9-positive monotypic fibrillary glomerulonephritis is not associated with monoclonal gammopathy in the vast majority of patients. Kidney international, 98(2), 498-504.

Nasr, S. H., Dasari, S., Lieske, J. C., Benson, L. M., Vanderboom, P. M., Holtz-Heppelmann, C. J., ... & Alexander, M. P. (2019). Serum levels of DNAJB9 are elevated in fibrillary glomerulonephritis patients. Kidney international, 95(5), 1269-1272.

Huang, Y., Arora, K., Mun, K. S., Yang, F., Moon, C., Yarlagadda, S., ... & Naren, A. P. (2019). Targeting DNAJB9, a novel ER luminal co-chaperone, to rescue ΔF508-CFTR. Scientific reports, 9(1), 1-11.

Nasr, S. H., Vrana, J. A., Dasari, S., Bridoux, F., Fidler, M. E., Kaaki, S., ... & Alexander, M. P. (2018). DNAJB9 is a specific immunohistochemical marker for fibrillary glomerulonephritis. Kidney international reports, 3(1), 56-64.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Mouse Anti-DNAJB9 Recombinant Antibody (CBFYE-0352) (CAT#: V2LY-0425-LY643)

Related Products

Mouse Anti-DNAJB9 Recombinant Antibody (CBFYE-0352) (CAT#: V2LY-0425-LY643)

Isotype control

For research use only. Not intended for any clinical use.

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