Mouse Anti-DNAJC5 Recombinant Antibody (CBMY-C0180) (CBMAB-V208-C0183-YY)

Name: Mouse Anti-DNAJC5 Recombinant Antibody (CBMY-C0180)
SKU: CBMAB-V208-C0183-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBMY-C0180

Application

ELISA, WB

Specificity

P. vivax

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

DnaJ Heat Shock Protein Family (Hsp40) Member C5; DnaJ (Hsp40) Homolog, Subfamily C, Member; Ceroid-Lipofuscinosis Neuronal Protein; CLN4; Ceroid-Lipofuscinosis, Neuronal 4 (Kufs Disease) 2
DnaJ Homolog Subfamily C Member; Cysteine String Protein Alpha 3; Cysteine String Protein 4

Introduction

DNAJC5 (DnaJ Heat Shock Protein Family (Hsp40) Member C5) is a Protein Coding gene. Diseases associated with DNAJC5 include Ceroid Lipofuscinosis, Neuronal, 4B, Autosomal Dominant and Ceroid Storage Disease.

Function

Acts as a general chaperone in regulated exocytosis (By similarity).

Acts as a co-chaperone for the SNARE protein SNAP-25 (By similarity).

Involved in the calcium-mediated control of a late stage of exocytosis (By similarity).

May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings (By similarity).

Biological Process

Chaperone-mediated protein folding Source: Ensembl
Exocytosis Source: ParkinsonsUK-UCL
Negative regulation of neuron apoptotic process Source: Ensembl
Regulated exocytosis Source: ParkinsonsUK-UCL
Regulation of synaptic vesicle cycle Source: Ensembl
Synaptic vesicle exocytosis Source: ParkinsonsUK-UCL

Cellular Location

Cell membrane; Cytosol; Membrane; Chromaffin granule membrane; Melanosome. The association with membranes is regulated by palmitoylation (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065).

Involvement in disease

Ceroid lipofuscinosis, neuronal, 4B (CLN4B):
An adult-onset neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. CLN4B has no visual involvement and is characterized by seizures and other neurologic symptoms.

PTM

Ser-10 phosphorylation induces an order-to-disorder transition triggering the interaction with Lys-58 (PubMed:27452402). This conformational switch modulates DNAJC5's cellular functions by reducing binding to syntaxin and synaptogamin without altering HSC70 interactions (PubMed:27452402).
Palmitoylated. Could be palmitoylated by DHHC3, DHHC7, DHHC15 and DHHC17. Palmitoylation occurs probably in the cysteine-rich domain and regulates DNAJC5 membrane attachment.

Lee, J., Xu, Y., Saidi, L., Xu, M., Zinsmaier, K., & Ye, Y. (2022). Abnormal triaging of misfolded proteins by adult neuronal ceroid lipofuscinosis-associated DNAJC5/CSPα mutants causes lipofuscin accumulation. Autophagy, 1-20.

Wu, S., Sirkis, D. W., & Schekman, R. (2022). Unconventional secretion of α-synuclein mediated by palmitoylated DNAJC5 oligomers. BioRxiv.

Huang, Q., Zhang, Y. F., Li, L. J., Dammer, E. B., Hu, Y. B., Xie, X. Y., ... & Ren, R. J. (2022). Adult-Onset Neuronal Ceroid Lipofuscinosis With a Novel DNAJC5 Mutation Exhibits Aberrant Protein Palmitoylation. Frontiers in aging neuroscience, 14.

Wang, H., Luo, J., Tian, X., Xu, L., Zhai, Z., Cheng, M., ... & Luo, S. (2021). DNAJC5 promotes hepatocellular carcinoma cells proliferation though regulating SKP2 mediated p27 degradation. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1868(6), 118994.

Faruq, R. N., D’Silva, P., Lau, F. D., Zhao, C., & Majumdar, S. (2021). Early-Onset Vascular Dementia in a 43-Year-Old Man with Accelerated Atherosclerotic Disease, Elevated Lipoprotein (a), and a Missense DNAJC5 Variant with Potential Association to Adult-Onset Ceroid Lipofuscinosis. Case reports in neurology, 13, 565-571.

Jedličková, I., Cadieux-Dion, M., Přistoupilová, A., Stránecký, V., Hartmannová, H., Hodaňová, K., ... & Kmoch, S. (2020). Autosomal-dominant adult neuronal ceroid lipofuscinosis caused by duplication in DNAJC5 initially missed by Sanger and whole-exome sequencing. European Journal of Human Genetics, 28(6), 783-789.

Xu, Y., Cui, L., Dibello, A., Wang, L., Lee, J., Saidi, L., ... & Ye, Y. (2018). DNAJC5 facilitates USP19-dependent unconventional secretion of misfolded cytosolic proteins. Cell discovery, 4(1), 1-18.

Valenzuela-Villatoro, M., García-Junco-Clemente, P., Nieto-González, J. L., & Fernández-Chacón, R. (2018). Presynaptic neurodegeneration: CSP-α/DNAJC5 at the synaptic vesicle cycle and beyond. Current Opinion in Physiology, 4, 65-69.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-DNAJC5 Recombinant Antibody (CBMY-C0180) (CAT#: V2LY-0425-LY659)

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Mouse Anti-DNAJC5 Recombinant Antibody (CBMY-C0180) (CAT#: V2LY-0425-LY659)

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Isotype control

For research use only. Not intended for any clinical use.

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