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Rabbit Anti-DNMT1 Recombinant Antibody (10E20) (CBMAB-D1403-YC)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
10E20
Antibody Isotype
IgG
Application
IHC-P, WB, IF

Basic Information

Immunogen
Synthetic peptide within human dnmt1 (aa1506-1549).
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
IF(ICC)1:50-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
BSA & Glycerol & TBS
Preservative
Sodium Azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Introduction
DNMT1 is an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE.
Entrez Gene ID
Human1786
Monkey574321
UniProt ID
HumanP26358
MonkeyA0A2K5UL02
Alternative Names
DNA Methyltransferase 1; DNA (Cytosine-5-)-Methyltransferase 1; CXXC-Type Zinc Finger Protein 9; DNA Methyltransferase HsaI; DNA MTase HsaI; EC 2.1.1.37; M.HsaI; CXXC9;
Function
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306).

Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306).

Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306).

Promotes tumor growth (PubMed:24623306).
Biological Process
Cellular response to amino acid stimulus Source: Ensembl
Chromatin organization Source: UniProtKB-KW
DNA methylation Source: ProtInc
DNA methylation involved in embryo development Source: Ensembl
Gene silencing Source: Ensembl
Maintenance of DNA methylation Source: UniProtKB
Negative regulation of gene expression Source: BHF-UCL
Negative regulation of histone H3-K9 methylation Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: ProtInc
Negative regulation of vascular associated smooth muscle cell apoptotic process Source: BHF-UCL
Negative regulation of vascular associated smooth muscle cell differentiation involved in phenotypic switching Source: BHF-UCL
Positive regulation of DNA methylation-dependent heterochromatin assembly Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of histone H3-K4 methylation Source: UniProtKB
Positive regulation of vascular associated smooth muscle cell proliferation Source: BHF-UCL
Ras protein signal transduction Source: UniProtKB
Cellular Location
Nucleus
Involvement in disease
Neuropathy, hereditary sensory, 1E (HSN1E):
A neurodegenerative disorder characterized by adult onset of progressive peripheral sensory loss associated with progressive hearing impairment and early-onset dementia.
Cerebellar ataxia, deafness, and narcolepsy, autosomal dominant (ADCADN):
An autosomal dominant neurologic disorder characterized by adult onset of progressive cerebellar ataxia, narcolepsy, cataplexy, sensorineural deafness, and dementia. More variable features include optic atrophy, sensory neuropathy, psychosis, and depression.
PTM
Sumoylated; sumoylation increases activity.
Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G2/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity.
Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability.
Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation.
Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.
More Infomation

Bayer, C., Pitschelatow, G., Hannemann, N., Linde, J., Reichard, J., Pensold, D., & Zimmer-Bensch, G. (2020). DNA Methyltransferase 1 (DNMT1) Acts on neurodegeneration by modulating proteostasis-relevant intracellular processes. International journal of molecular sciences, 21(15), 5420.

Wang, L., Lee, J. Y., Gao, L., Yin, J., Duan, Y., Jimenez, L. A., ... & Zhong, W. (2019). A DNA aptamer for binding and inhibition of DNA methyltransferase 1. Nucleic acids research, 47(22), 11527-11537.

Chen, L., Chan, L. S., Lung, H. L., Yip, T. T. C., Ngan, R. K. C., Wong, J. W. C., ... & Mak, N. K. (2019). Crucifera sulforaphane (SFN) inhibits the growth of nasopharyngeal carcinoma through DNA methyltransferase 1 (DNMT1)/Wnt inhibitory factor 1 (WIF1) axis. Phytomedicine, 63, 153058.

Li, Y., Chen, F., Chu, J., Wu, C., Li, Y., Li, H., & Ma, H. (2019). miR-148-3p inhibits growth of glioblastoma targeting DNA methyltransferase-1 (DNMT1). Oncology Research, 27(8), 911.

Yarychkivska, O., Shahabuddin, Z., Comfort, N., Boulard, M., & Bestor, T. H. (2018). BAH domains and a histone-like motif in DNA methyltransferase 1 (DNMT1) regulate de novo and maintenance methylation in vivo. Journal of Biological Chemistry, 293(50), 19466-19475.

Jung, H. J., Byun, H. O., Jee, B. A., Min, S., Jeoun, U. W., Lee, Y. K., ... & Yoon, G. (2017). The ubiquitin-like with PHD and ring finger domains 1 (UHRF1)/DNA methyltransferase 1 (DNMT1) axis is a primary regulator of cell senescence. Journal of Biological Chemistry, 292(9), 3729-3739.

Saini, S. K., Mangalhara, K. C., Prakasam, G., & Bamezai, R. N. K. (2017). DNA Methyltransferase1 (DNMT1) Isoform3 methylates mitochondrial genome and modulates its biology. Scientific reports, 7(1), 1-10.

Krishna, S., Shukla, S., Lakra, A. D., Meeran, S. M., & Siddiqi, M. I. (2017). Identification of potent inhibitors of DNA methyltransferase 1 (DNMT1) through a pharmacophore-based virtual screening approach. Journal of Molecular Graphics and Modelling, 75, 174-188.

Cai, Y., Tsai, H. C., Yen, R. W. C., Zhang, Y. W., Kong, X., Wang, W., ... & Baylin, S. B. (2017). Critical threshold levels of DNA methyltransferase 1 are required to maintain DNA methylation across the genome in human cancer cells. Genome research, 27(4), 533-544.

Pensold, D., Symmank, J., Hahn, A., Lingner, T., Salinas-Riester, G., Downie, B. R., ... & Zimmer, G. (2017). The DNA methyltransferase 1 (DNMT1) controls the shape and dynamics of migrating POA-derived interneurons fated for the murine cerebral cortex. Cerebral Cortex, 27(12), 5696-5714.

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For research use only. Not intended for any clinical use.

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