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Mouse Anti-dok1 Recombinant Antibody (P0282) (CBMAB-P0282-YC)


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Published Data
Mouse Anti-dok1 Recombinant Antibody (P0282) (CBMAB-P0282-YC-1) in WB
Mouse Anti-dok1 Recombinant Antibody (P0282) (CBMAB-P0282-YC-1) in WB
Lysates from the BCR-ABL expressing 32D cell lines, control line K562 and parental 32D cells were immunoprecipitated (IP) for the indicated proteins and blotted to determine interactions between BCR-ABL and p62DOK. ...View More
Johnson, K. J., Griswold, I. J., O'Hare, T., Corbin, A. S., Loriaux, M., Deininger, M. W., & Druker, B. J. (2009). A BCR-ABL mutant lacking direct binding sites for the GRB2, CBL and CRKL adapter proteins fails to induce leukemia in mice. PloS one, 4(10), e7439.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
P0282
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, IHC-P

Basic Information

Immunogen
Amino acids 1-276 mapping at the N-terminus of mouse Dok-1.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Docking Protein 1
Introduction
dok1 is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes.
Entrez Gene ID
Human1796
Mouse13448
Rat312477
UniProt ID
HumanQ99704
MouseP97465
RatQ4QQV2
Alternative Names
Docking Protein 1; Downstream Of Tyrosine Kinase 1; Pp62; Docking Protein 1, 62kDa (Downstream Of Tyrosine Kinase 1); Docking Protein 1, 62kD (Downstream Of Tyrosine Kinase 1); P62(Dok); P62DOK;
Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
Biological Process
Cell surface receptor signaling pathway Source: ProtInc
Macrophage colony-stimulating factor signaling pathway Source: Ensembl
Ras protein signal transduction Source: Ensembl
Signal transduction Source: ProtInc
Transmembrane receptor protein tyrosine kinase signaling pathway Source: ProtInc
Cellular Location
Isoform 1: Nucleus; Cytoplasm
Isoform 3: Perinuclear region
PTM
Constitutively tyrosine-phosphorylated. Phosphorylated by TEC (By similarity). Phosphorylated by LYN (By similarity). Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. Phosphorylated on tyrosine residues by SRMS.
More Infomation

Ha, X., Cai, X., Cao, H., Li, J., Yang, B., Jiang, R., ... & Xin, Y. (2021). Docking protein 1 and free fatty acids are associated with insulin resistance in patients with type 2 diabetes mellitus. Journal of International Medical Research, 49(11), 03000605211048293.

Gutting, T., Merkel, A., Fink, D. J., Hetjens, S., Weidner, P., Yu, Y., ... & Belle, S. (2021). Expression of the EGFR-RAS Inhibitory Proteins DOK1 and MTMR7 and its Significance in Colorectal Adenoma and Adenoma Recurrence. Journal of Gastrointestinal & Liver Diseases, 30(4).

Tuna, E., Ersoy, Y. E., Bulut, P., Ozdemir, F., & Buyru, N. (2020). Analysis of the DOK1 gene in breast cancer. Molecular biology reports, 47(3), 1605-1612.

Merkel, A., Hetjens, S., Weidner, P., Gaiser, T., Belle, S., Ebert, M. P., ... & Gutting, T. (2020). Der Verlust des RAS-Inhibitors Docking Protein 1 (DOK1) ist ein häufiges Ereignis in serratierten kolorektalen Läsionen. Zeitschrift für Gastroenterologie, 58(08), P-013.

Li, T., Li, B., Sara, A., Ay, C., Leung, W. Y., Zhang, Y., ... & Burgermeister, E. (2019). Docking protein-1 promotes inflammatory macrophage signaling in gastric cancer. Oncoimmunology, 8(11), e1649961.

Choi, E. J., Lee, J. H., Kim, M. S., Song, S. Y., Yoo, N. J., & Lee, S. H. (2018). Intratumoral heterogeneity of somatic mutations for NRIP1, DOK1, ULK1, ULK2, DLGAP3, PARD3 and PRKCI in colon cancers. Pathology & Oncology Research, 24(4), 827-832.

Chatterjee, D., D’Souza, A., Zhang, Y., Bin, W., Tan, S. M., & Bhattacharjya, S. (2018). Interaction analyses of 14-3-3ζ, Dok1, and phosphorylated integrin β cytoplasmic tails reveal a bi-molecular switch in integrin regulation. Journal of molecular biology, 430(21), 4419-4430.

Ding, X., Wang, W., Wang, M., Wu, J., & Yao, F. (2017). DOK1/PPARgamma pathway mediates anti-tumor ability of all-trans retinoic acid in breast cancer MCF-7 cells. Biochemical and biophysical research communications, 487(2), 189-193.

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For research use only. Not intended for any clinical use.

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