Mouse Anti-dok1 Recombinant Antibody (P0282) (CBMAB-P0282-YC)

Name: Mouse Anti-dok1 Recombinant Antibody (P0282)
SKU: CBMAB-P0282-YC
Rating: 5 (1 reviews)

See all dok1 antibodies

Compare Online Inquiry

Published Data

Mouse Anti-dok1 Recombinant Antibody (P0282) (CBMAB-P0282-YC-1) in WB

Lysates from the BCR-ABL expressing 32D cell lines, control line K562 and parental 32D cells were immunoprecipitated (IP) for the indicated proteins and blotted to determine interactions between BCR-ABL and p62DOK. ...View More

Johnson, K. J., Griswold, I. J., O'Hare, T., Corbin, A. S., Loriaux, M., Deininger, M. W., & Druker, B. J. (2009). A BCR-ABL mutant lacking direct binding sites for the GRB2, CBL and CRKL adapter proteins fails to induce leukemia in mice. PloS one, 4(10), e7439.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

P0282

Application

WB, IP, IF, IHC-P

Immunogen

Amino acids 1-276 mapping at the N-terminus of mouse Dok-1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Docking Protein 1

Introduction

dok1 is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes.

Entrez Gene ID

Human	1796
Mouse	13448
Rat	312477

UniProt ID

Human	Q99704
Mouse	P97465
Rat	Q4QQV2

Alternative Names

Docking Protein 1; Downstream Of Tyrosine Kinase 1; Pp62; Docking Protein 1, 62kDa (Downstream Of Tyrosine Kinase 1); Docking Protein 1, 62kD (Downstream Of Tyrosine Kinase 1); P62(Dok); P62DOK;

Function

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.

Biological Process

Cell surface receptor signaling pathway Source: ProtInc
Macrophage colony-stimulating factor signaling pathway Source: Ensembl
Ras protein signal transduction Source: Ensembl
Signal transduction Source: ProtInc
Transmembrane receptor protein tyrosine kinase signaling pathway Source: ProtInc

Cellular Location

Isoform 1: Nucleus; Cytoplasm
Isoform 3: Perinuclear region

PTM

Constitutively tyrosine-phosphorylated. Phosphorylated by TEC (By similarity). Phosphorylated by LYN (By similarity). Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. Phosphorylated on tyrosine residues by SRMS.

Ha, X., Cai, X., Cao, H., Li, J., Yang, B., Jiang, R., ... & Xin, Y. (2021). Docking protein 1 and free fatty acids are associated with insulin resistance in patients with type 2 diabetes mellitus. Journal of International Medical Research, 49(11), 03000605211048293.

Gutting, T., Merkel, A., Fink, D. J., Hetjens, S., Weidner, P., Yu, Y., ... & Belle, S. (2021). Expression of the EGFR-RAS Inhibitory Proteins DOK1 and MTMR7 and its Significance in Colorectal Adenoma and Adenoma Recurrence. Journal of Gastrointestinal & Liver Diseases, 30(4).

Tuna, E., Ersoy, Y. E., Bulut, P., Ozdemir, F., & Buyru, N. (2020). Analysis of the DOK1 gene in breast cancer. Molecular biology reports, 47(3), 1605-1612.

Merkel, A., Hetjens, S., Weidner, P., Gaiser, T., Belle, S., Ebert, M. P., ... & Gutting, T. (2020). Der Verlust des RAS-Inhibitors Docking Protein 1 (DOK1) ist ein häufiges Ereignis in serratierten kolorektalen Läsionen. Zeitschrift für Gastroenterologie, 58(08), P-013.

Li, T., Li, B., Sara, A., Ay, C., Leung, W. Y., Zhang, Y., ... & Burgermeister, E. (2019). Docking protein-1 promotes inflammatory macrophage signaling in gastric cancer. Oncoimmunology, 8(11), e1649961.

Choi, E. J., Lee, J. H., Kim, M. S., Song, S. Y., Yoo, N. J., & Lee, S. H. (2018). Intratumoral heterogeneity of somatic mutations for NRIP1, DOK1, ULK1, ULK2, DLGAP3, PARD3 and PRKCI in colon cancers. Pathology & Oncology Research, 24(4), 827-832.

Chatterjee, D., D’Souza, A., Zhang, Y., Bin, W., Tan, S. M., & Bhattacharjya, S. (2018). Interaction analyses of 14-3-3ζ, Dok1, and phosphorylated integrin β cytoplasmic tails reveal a bi-molecular switch in integrin regulation. Journal of molecular biology, 430(21), 4419-4430.

Ding, X., Wang, W., Wang, M., Wu, J., & Yao, F. (2017). DOK1/PPARgamma pathway mediates anti-tumor ability of all-trans retinoic acid in breast cancer MCF-7 cells. Biochemical and biophysical research communications, 487(2), 189-193.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-dok1 Recombinant Antibody (P0282)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Rabbit Anti-DOK1 Recombinant Antibody (EG986) (CAT#: V2LY-0425-LY784)

Rabbit Anti-DOK1 Recombinant Antibody (EG985) (CAT#: V2LY-0425-LY783)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry