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Rabbit Anti-DOK1 Recombinant Antibody (EG985) (V2LY-0425-LY783)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG985
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Recombinant protein within human dok1 (aa 360-481).
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
BSA & Glycerol & TBS
Preservative
Sodium Azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Docking Protein 1
Entrez Gene ID
Human1796
Mouse13448
Rat312477
UniProt ID
HumanQ99704
MouseP97465
RatQ4QQV2
Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
Biological Process
Cell surface receptor signaling pathway Source: ProtInc
Macrophage colony-stimulating factor signaling pathway Source: Ensembl
Ras protein signal transduction Source: Ensembl
Signal transduction Source: ProtInc
Transmembrane receptor protein tyrosine kinase signaling pathway Source: ProtInc
Cellular Location
Isoform 1: Nucleus; Cytoplasm
Isoform 3: Perinuclear region
PTM
Constitutively tyrosine-phosphorylated. Phosphorylated by TEC (By similarity). Phosphorylated by LYN (By similarity). Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. Phosphorylated on tyrosine residues by SRMS.
More Infomation

Ha, X., Cai, X., Cao, H., Li, J., Yang, B., Jiang, R., ... & Xin, Y. (2021). Docking protein 1 and free fatty acids are associated with insulin resistance in patients with type 2 diabetes mellitus. Journal of International Medical Research, 49(11), 03000605211048293.

Gutting, T., Merkel, A., Fink, D. J., Hetjens, S., Weidner, P., Yu, Y., ... & Belle, S. (2021). Expression of the EGFR-RAS Inhibitory Proteins DOK1 and MTMR7 and its Significance in Colorectal Adenoma and Adenoma Recurrence. Journal of Gastrointestinal & Liver Diseases, 30(4).

Tuna, E., Ersoy, Y. E., Bulut, P., Ozdemir, F., & Buyru, N. (2020). Analysis of the DOK1 gene in breast cancer. Molecular biology reports, 47(3), 1605-1612.

Merkel, A., Hetjens, S., Weidner, P., Gaiser, T., Belle, S., Ebert, M. P., ... & Gutting, T. (2020). Der Verlust des RAS-Inhibitors Docking Protein 1 (DOK1) ist ein häufiges Ereignis in serratierten kolorektalen Läsionen. Zeitschrift für Gastroenterologie, 58(08), P-013.

Li, T., Li, B., Sara, A., Ay, C., Leung, W. Y., Zhang, Y., ... & Burgermeister, E. (2019). Docking protein-1 promotes inflammatory macrophage signaling in gastric cancer. Oncoimmunology, 8(11), e1649961.

Choi, E. J., Lee, J. H., Kim, M. S., Song, S. Y., Yoo, N. J., & Lee, S. H. (2018). Intratumoral heterogeneity of somatic mutations for NRIP1, DOK1, ULK1, ULK2, DLGAP3, PARD3 and PRKCI in colon cancers. Pathology & Oncology Research, 24(4), 827-832.

Chatterjee, D., D’Souza, A., Zhang, Y., Bin, W., Tan, S. M., & Bhattacharjya, S. (2018). Interaction analyses of 14-3-3ζ, Dok1, and phosphorylated integrin β cytoplasmic tails reveal a bi-molecular switch in integrin regulation. Journal of molecular biology, 430(21), 4419-4430.

Ding, X., Wang, W., Wang, M., Wu, J., & Yao, F. (2017). DOK1/PPARgamma pathway mediates anti-tumor ability of all-trans retinoic acid in breast cancer MCF-7 cells. Biochemical and biophysical research communications, 487(2), 189-193.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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