Mouse Anti-DPEP3 Recombinant Antibody (D1543) (V2LY-0425-LY814)

Name: Mouse Anti-DPEP3 Recombinant Antibody (D1543)
SKU: V2LY-0425-LY814
Rating: 5 (1 reviews)

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Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

D1543

Antibody Isotype

IgM, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 46-67 near the N-terminus of human DPEP3.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgM, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

dipeptidase 3

Entrez Gene ID

64180

UniProt ID

Q9H4B8

Function

Dipeptidase that hydrolyzes cystinyl-bis-glycine. Does not hydrolyze leukotriene D4 (LTD4) into leukotriene E4 (LTE4).

Cellular Location

Membrane

More Infomation

References

Hayashi, K., Longenecker, K. L., Koenig, P., Prashar, A., Hampl, J., Stoll, V., & Vivona, S. (2020). Structure of human DPEP3 in complex with the SC-003 antibody Fab fragment reveals basis for lack of dipeptidase activity. Journal of Structural Biology, 211(1), 107512.

Xie, Y., Khan, R., Wahab, F., Hussain, H. M. J., Ali, A., Ma, H., ... & Shi, Q. (2019). The testis-specifically expressed Dpep3 is not essential for male fertility in mice. Gene, 711, 143925.

Schiza, C., Korbakis, D., Panteleli, E., Jarvi, K., Drabovich, A. P., & Diamandis, E. P. (2018). Discovery of a human testis-specific protein complex TEX101-DPEP3 and selection of its disrupting antibodies. Molecular & Cellular Proteomics, 17(12), 2480-2495.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-DPEP3 Recombinant Antibody (F-3)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

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