Rat Anti-DPPA3 Recombinant Antibody (CBXS-1750) (V2LY-0425-LY879)

Name: Rat Anti-DPPA3 Recombinant Antibody (CBXS-1750)
SKU: V2LY-0425-LY879
Rating: 5 (1 reviews)

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Published Data

Rat Anti-DPPA3 Recombinant Antibody (CBXS-1750) (V2LY-0425-LY879-1) in WB

Western blot analysis of Dppa3 protein level in Dppa3 OE and Con ESCs. Right: Quantification of relative Dppa3 protein levels normalized to Tubulin by ImageJ software (n = 3; *p < 0.05). ...View More

Zhang, C., Wen, H., Liu, S., Fu, E., Yu, L., Chen, S., ... & Liu, N. (2022). Maternal factor Dppa3 activates 2C-like genes and depresses DNA methylation in mouse embryonic stem cells. Frontiers in cell and developmental biology, 10, 882671.

Rat Anti-DPPA3 Recombinant Antibody (CBXS-1750) (V2LY-0425-LY879-2) in IF

Dppa3 overexpression increases Zscan4 positive cells by immunofluorescence staining of Zscan4 (red) and Dppa3 (green) in stable Dppa3 overexpressed D3 ESCs. Scale bar represents 10 μm. ...View More

Rat Anti-DPPA3 Recombinant Antibody (CBXS-1750) (V2LY-0425-LY879-3) in FC

FACS profiles of Zscan4+ ESCs population in stable Dppa3 OE ESCs. ...View More

Datasheet

Summary

Host Animal

Rat

Specificity

Human, Mouse

Clone

CBXS-1750

Antibody Isotype

IgG2a

Application

WB, IF

Basic Information

Immunogen

Recombinant mouse Stella/Dppa3 (Met1-Asn150).

Host Species

Rat

Specificity

Human, Mouse

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1 µg/ml
IF(ICC)	8-25 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

Trehalose & PBS

Preservative

None

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

developmental pluripotency-associated 3

Entrez Gene ID

73708

UniProt ID

Q8QZY3

Function

Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis (By similarity).

Biological Process

Chromatin organization Source: UniProtKB-KW
Negative regulation of DNA demethylation Source: UniProtKB
Protection of DNA demethylation of female pronucleus Source: UniProtKB
Regulation of genetic imprinting Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm. Mainly localizes in the female pronucleus, localization to the male pronucleus in much weaker.

More Infomation

References

Zhao, S., Zhang, C., Xu, J., Liu, S., Yu, L., Chen, S., ... & Liu, N. (2022). Dppa3 facilitates self-renewal of embryonic stem cells by stabilization of pluripotent factors. Stem cell research & therapy, 13(1), 1-12.

Mulholland, C. B., Nishiyama, A., Ryan, J., Nakamura, R., Yiğit, M., Glück, I. M., ... & Leonhardt, H. (2020). Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive DNA demethylation in mammals. Nature communications, 11(1), 1-24.

Wang, Q. Q., Zhang, Y. M., Zhong, X., Li, J. W., An, X. R., & Hou, J. (2019). Dimethylated histone H3 lysine 9 is dispensable for the interaction between developmental pluripotency-associated protein 3 (Dppa3) and ten-eleven translocation 3 (Tet3) in somatic cells. Reproduction, Fertility and Development, 31(2), 347-356.

Zhao, S., Xu, J., Liu, S., Cui, K., Li, Z., & Liu, N. (2019). Dppa3 in pluripotency maintenance of ES cells and early embryogenesis. Journal of Cellular Biochemistry, 120(4), 4794-4799.

Yan, Q., Zhang, Y., Yu, D., Kam, N. W., & Guan, X. Y. (2018). Characterization of the role of DPPA3 in stemness regulation in hepatocellular carcinoma by DNA methylation.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Alternative Versions

Rat Anti-DPPA3 Recombinant Antibody (CBXS-1750)

Related Products

Mouse Anti-DPPA3 Recombinant Antibody (CBYCD-552)

Rat Anti-DPPA3 Recombinant Antibody (CBXS-1750)

Rat Anti-DPPA3 Recombinant Antibody (CBYCD-552)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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