Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Rat Anti-DPPA3 Recombinant Antibody (CBXS-1750) (V2LY-0425-LY879)

Online Inquiry
Published Data

Summary

Host Animal
Rat
Specificity
Human, Mouse
Clone
CBXS-1750
Antibody Isotype
IgG2a
Application
WB, IF

Basic Information

Immunogen
Recombinant mouse Stella/Dppa3 (Met1-Asn150).
Host Species
Rat
Specificity
Human, Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1 µg/ml
IF(ICC)8-25 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
Trehalose & PBS
Preservative
None
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
developmental pluripotency-associated 3
Entrez Gene ID
UniProt ID
Function
Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis (By similarity).
Biological Process
Chromatin organization Source: UniProtKB-KW
Negative regulation of DNA demethylation Source: UniProtKB
Protection of DNA demethylation of female pronucleus Source: UniProtKB
Regulation of genetic imprinting Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm. Mainly localizes in the female pronucleus, localization to the male pronucleus in much weaker.
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As
Loading...
Have you used Rat Anti-DPPA3 Recombinant Antibody (CBXS-1750)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Online Inquiry