Rat Anti-DPPA3 Recombinant Antibody (CBYCD-552) (CBMAB-D1627-YC)

Provided herein is a Rat monoclonal antibody, which binds to Developmental Pluripotency Associated 3 (DPPA3). The antibody can be used for immunoassay techniques, such as ICC, IF, WB.
See all DPPA3 antibodies

Datasheet

Summary

Host Animal

Rat

Specificity

Mouse

Clone

CBYCD-552

Antibody Isotype

IgG2a

Application

ICC, IF, WB

Basic Information

Immunogen

E. coli-derived recombinant mouse Stella/Dppa3 Met1-Asn150

Specificity

Mouse

Antibody Isotype

IgG2a

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

developmental pluripotency-associated 3

Introduction

DPPA3 is a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice, this gene may play a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality. In humans, related intronless loci are located on chromosomes 14 and X.

Entrez Gene ID

73708

UniProt ID

Q8QZY3

Alternative Names

Developmental Pluripotency Associated 3; Stella-Related Protein; Developmental Pluripotency-Associated Protein 3; STELLAR; STELLA; Pgc7;

Function

Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis (By similarity).

Biological Process

Chromatin organization Source: UniProtKB-KW
Negative regulation of DNA demethylation Source: UniProtKB
Protection of DNA demethylation of female pronucleus Source: UniProtKB
Regulation of genetic imprinting Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm. Mainly localizes in the female pronucleus, localization to the male pronucleus in much weaker.

References

Zhao, S., Zhang, C., Xu, J., Liu, S., Yu, L., Chen, S., ... & Liu, N. (2022). Dppa3 facilitates self-renewal of embryonic stem cells by stabilization of pluripotent factors. Stem cell research & therapy, 13(1), 1-12.

Mulholland, C. B., Nishiyama, A., Ryan, J., Nakamura, R., Yiğit, M., Glück, I. M., ... & Leonhardt, H. (2020). Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive DNA demethylation in mammals. Nature communications, 11(1), 1-24.

Wang, Q. Q., Zhang, Y. M., Zhong, X., Li, J. W., An, X. R., & Hou, J. (2019). Dimethylated histone H3 lysine 9 is dispensable for the interaction between developmental pluripotency-associated protein 3 (Dppa3) and ten-eleven translocation 3 (Tet3) in somatic cells. Reproduction, Fertility and Development, 31(2), 347-356.

Zhao, S., Xu, J., Liu, S., Cui, K., Li, Z., & Liu, N. (2019). Dppa3 in pluripotency maintenance of ES cells and early embryogenesis. Journal of Cellular Biochemistry, 120(4), 4794-4799.

Yan, Q., Zhang, Y., Yu, D., Kam, N. W., & Guan, X. Y. (2018). Characterization of the role of DPPA3 in stemness regulation in hepatocellular carcinoma by DNA methylation.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Rat Anti-DPPA3 Recombinant Antibody (CBYCD-552)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Rat Anti-DPPA3 Recombinant Antibody (CBXS-1750)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: