Mouse Anti-DPPA4 Recombinant Antibody (1C1) (V2LY-0425-LY881)

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Basic Information

Host Animal
Mouse
Clone
1C1
Application
IF, WB
Immunogen
Human recombinant protein fragment corresponding to amino acids 9-254 of human DPPA4 produced in E.coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:2,000
IF(ICC)1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
BSA & Glycerol & PBS
Preservative
Sodium Azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Developmental Pluripotency Associated 4
Entrez Gene ID
UniProt ID
Function
May be involved in the maintenance of active epigenetic status of target genes. May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
Biological Process
Lung-associated mesenchyme development Source: Ensembl
System development Source: GO_Central
Cellular Location
Nucleus. Associated with transcriptionally active chromatin.

Auvinen, P., Vehviläinen, J., Marjonen, H., Modhukur, V., Sokka, J., Wallén, E., ... & Kaminen-Ahola, N. (2022). Chromatin remodeler developmental pluripotency associated factor 4 (DPPA4) is a candidate gene for alcohol-induced developmental disorders. medRxiv.

Kubinyecz, O., Santos, F., Drage, D., Reik, W., & Eckersley-Maslin, M. A. (2021). Maternal Dppa2 and Dppa4 are dispensable for zygotic genome activation but important for offspring survival. Development, 148(24), dev200191.

Klein, R. H., & Knoepfler, P. S. (2021). DPPA2, DPPA4, and other DPPA factor epigenomic functions in cell fate and cancer. Stem cell reports, 16(12), 2844-2851.

Gretarsson, K. H., & Hackett, J. A. (2020). Dppa2 and Dppa4 counteract de novo methylation to establish a permissive epigenome for development. Nature Structural & Molecular Biology, 27(8), 706-716.

De Iaco, A., Coudray, A., Duc, J., & Trono, D. (2019). DPPA2 and DPPA4 are necessary to establish a 2C‐like state in mouse embryonic stem cells. EMBO reports, 20(5), e47382.

Li, L., Wang, Y., Wang, Q., Qu, J., Wei, X., Xu, J., ... & Zhang, Y. (2019). High developmental pluripotency‑associated 4 expression promotes cell proliferation and glycolysis, and predicts poor prognosis in non‑small‑cell lung cancer. Molecular Medicine Reports, 20(1), 445-454.

Eckersley-Maslin, M., Alda-Catalinas, C., Blotenburg, M., Kreibich, E., Krueger, C., & Reik, W. (2019). Dppa2 and Dppa4 directly regulate the Dux-driven zygotic transcriptional program. Genes & development, 33(3-4), 194-208.

Klein, R. H., Tung, P. Y., Somanath, P., Fehling, H. J., & Knoepfler, P. S. (2018). Genomic functions of developmental pluripotency associated factor 4 (Dppa4) in pluripotent stem cells and cancer. Stem cell research, 31, 83-94.

Somanath, P., Bush, K. M., & Knoepfler, P. S. (2018). ERBB3-binding protein 1 (EBP1) is a novel developmental pluripotency-associated-4 (DPPA4) cofactor in human pluripotent cells. Stem Cells, 36(5), 671-682.

Jabbar, S., Belden, W., Gangisetty, O., & Sarkar, D. K. (2017). Developmental pluripotency associated 4: A novel putative predictor for prognosis of aggressive prolactin secreting tumors in the pituitary.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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