Mouse Anti-DPPA4 Recombinant Antibody (2F6) (CBMAB-D1628-YC)

Name: Mouse Anti-DPPA4 Recombinant Antibody (2F6)
SKU: CBMAB-D1628-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2F6

Application

FC, WB

Immunogen

Human recombinant protein fragment corresponding to amino acids 9-254 of human DPPA4 produced in E.coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & Glycerol & PBS

Preservative

Sodium Azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Developmental Pluripotency Associated 4

Introduction

DPPA4 is a nuclear factor that is involved in the maintenance of pluripotency in stem cells and essential for embryogenesis. The encoded protein has a scaffold-attachment factor A/B, acinus and PIAS (SAP) domain that binds DNA and is thought to modify chromatin. Mice with a homozygous knockout of the orthologous gene die during late embryonic development or within hours after birth. Knockout embryos are normal in size at embryonic day 18.5 but exhibit skeletal and lung tissue abnormalities. This gene, when mutated, is highly expressed in embryonal carcinomas, pluripotent germ cell tumors, and other cancers and is thought to play an important role in tumor progression. Multiple pseudogenes of this gene have been identified.

Entrez Gene ID

55211

UniProt ID

Q7L190

Alternative Names

Developmental Pluripotency Associated 4; Developmental Pluripotency-Associated Protein 4; 2410091M23Rik;

Function

May be involved in the maintenance of active epigenetic status of target genes. May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.

Biological Process

Lung-associated mesenchyme development Source: Ensembl
System development Source: GO_Central

Cellular Location

Nucleus. Associated with transcriptionally active chromatin.

Auvinen, P., Vehviläinen, J., Marjonen, H., Modhukur, V., Sokka, J., Wallén, E., ... & Kaminen-Ahola, N. (2022). Chromatin remodeler developmental pluripotency associated factor 4 (DPPA4) is a candidate gene for alcohol-induced developmental disorders. medRxiv.

Kubinyecz, O., Santos, F., Drage, D., Reik, W., & Eckersley-Maslin, M. A. (2021). Maternal Dppa2 and Dppa4 are dispensable for zygotic genome activation but important for offspring survival. Development, 148(24), dev200191.

Klein, R. H., & Knoepfler, P. S. (2021). DPPA2, DPPA4, and other DPPA factor epigenomic functions in cell fate and cancer. Stem cell reports, 16(12), 2844-2851.

Gretarsson, K. H., & Hackett, J. A. (2020). Dppa2 and Dppa4 counteract de novo methylation to establish a permissive epigenome for development. Nature Structural & Molecular Biology, 27(8), 706-716.

De Iaco, A., Coudray, A., Duc, J., & Trono, D. (2019). DPPA2 and DPPA4 are necessary to establish a 2C‐like state in mouse embryonic stem cells. EMBO reports, 20(5), e47382.

Li, L., Wang, Y., Wang, Q., Qu, J., Wei, X., Xu, J., ... & Zhang, Y. (2019). High developmental pluripotency‑associated 4 expression promotes cell proliferation and glycolysis, and predicts poor prognosis in non‑small‑cell lung cancer. Molecular Medicine Reports, 20(1), 445-454.

Eckersley-Maslin, M., Alda-Catalinas, C., Blotenburg, M., Kreibich, E., Krueger, C., & Reik, W. (2019). Dppa2 and Dppa4 directly regulate the Dux-driven zygotic transcriptional program. Genes & development, 33(3-4), 194-208.

Klein, R. H., Tung, P. Y., Somanath, P., Fehling, H. J., & Knoepfler, P. S. (2018). Genomic functions of developmental pluripotency associated factor 4 (Dppa4) in pluripotent stem cells and cancer. Stem cell research, 31, 83-94.

Somanath, P., Bush, K. M., & Knoepfler, P. S. (2018). ERBB3-binding protein 1 (EBP1) is a novel developmental pluripotency-associated-4 (DPPA4) cofactor in human pluripotent cells. Stem Cells, 36(5), 671-682.

Jabbar, S., Belden, W., Gangisetty, O., & Sarkar, D. K. (2017). Developmental pluripotency associated 4: A novel putative predictor for prognosis of aggressive prolactin secreting tumors in the pituitary.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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