Mouse Anti-DPYSL3 Recombinant Antibody (9G340) (CBMAB-D1657-YC)

Name: Mouse Anti-DPYSL3 Recombinant Antibody (9G340)
SKU: CBMAB-D1657-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DPYSL3 Recombinant Antibody (9G340) (CBMAB-D1657-YC-1) in WB

C4-2 and LNCaP cells were seeded in 6-well plates and the RNA conjugates were added into cell culture for 3 days as indicated (concentration at nM). Cells were harvested for western blot assays with anti-CRMP4 antibodies. Actin blot served as protein loading control. ...View More

Li, C., Jiang, W., Hu, Q., Li, L. C., Dong, L., Chen, R., ... & Li, B. (2016). Enhancing DPYSL3 gene expression via a promoter-targeted small activating RNA approach suppresses cancer cell motility and metastasis. Oncotarget, 7(16), 22893.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

9G340

Application

ELISA, WB, IP

Immunogen

Recombinant human CRMP-4.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

dihydropyrimidinase-like 3

Introduction

DPYSL3 (Dihydropyrimidinase Like 3) is a protein coding gene.Diseases associated with DPYSL3 include Prostate Lymphoma and Hypertensive Encephalopathy. Among its related pathways are Semaphorin interactions and Developmental Biology. It has proved that the protein has hydrolase activity and phosphoprotein binding. And a paralog is CRMP1.

Entrez Gene ID

Human	1809
Mouse	22240
Rat	25418

UniProt ID

Human	Q14195
Mouse	Q62188
Rat	Q62952

Alternative Names

Dihydropyrimidinase Like 3; Unc-33-Like Phosphoprotein 1; CRMP-4; ULIP-1; CRMP4; DRP-3; DRP3; ULIP;

Function

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration (By similarity).

Biological Process

Actin crosslink formation Source: UniProtKB
Actin filament bundle assembly Source: UniProtKB
Cellular response to cytokine stimulus Source: UniProtKB
Negative regulation of cell migration Source: UniProtKB
Negative regulation of neuron projection development Source: UniProtKB
Neuron development Source: Ensembl
Positive regulation of filopodium assembly Source: UniProtKB
Positive regulation of neuron projection development Source: UniProtKB
Response to axon injury Source: UniProtKB

Cellular Location

Cytoplasm; Growth cone. Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone.

PTM

Phosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr-514 and Ser-518 by GSK3.

Zhong, H., & Luo, X. (2021). Serum Dihydropyrimidinase-Like 3 Concentration in Patients with Gastric Cancer and Its Diagnostic Value. Iranian Journal of Public Health, 50(9), 1789.

Tsai, Y. M., Wu, K. L., Chang, Y. Y., Hung, J. Y., Chang, W. A., Chang, C. Y., ... & Hsu, Y. L. (2020). Upregulation of Thr/Tyr kinase increases the cancer progression by neurotensin and dihydropyrimidinase-Like 3 in lung cancer. International Journal of Molecular Sciences, 21(5), 1640.

Umetsu, S., Mizukami, H., Saito, T., Uchida, C., Igawa, A., Kudo, K., ... & Hakamada, K. (2020). Diabetes, an independent poor prognostic factor of non-B non-C hepatocellular carcinoma, correlates with dihydropyrimidinase-like 3 promoter methylation. Scientific reports, 10(1), 1-11.

Mamoor, S. (2020). Dihydropyrimidinase like 3 (DPYSL3) is differentially expressed in metastatic breast cancer, both in metastases to the brain and to the lymph nodes.

Matsunuma, R., Chan, D. W., Kim, B. J., Singh, P., Han, A., Saltzman, A., ... & Ellis, M. J. (2019). Abstract P5-08-01: DPYSL3 modulates mitosis, migration and epithelial to mesenchymal transition in claudin-low breast cancer.

Matsunuma, R., Chan, D. W., Kim, B. J., Singh, P., Han, A., Saltzman, A. B., ... & Ellis, M. J. (2018). DPYSL3 modulates mitosis, migration, and epithelial-to-mesenchymal transition in claudin-low breast cancer. Proceedings of the National Academy of Sciences, 115(51), E11978-E11987.

Yang, Y., Jiang, Y., Xie, D., Liu, M., Song, N., Zhu, J., ... & Zhu, C. (2018). Inhibition of cell-adhesion protein DPYSL3 promotes metastasis of lung cancer. Respiratory research, 19(1), 1-7.

Matsunuma, R., Chan, D. W., Kim, B. J., Singh, P., Han, A., Malovannaya, A., ... & Ellis, M. J. (2018). Abstract P1-01-08: DPYSL3 modulates proliferation and migration in claudin-low breast cancer.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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