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Mouse Anti-DR1 Recombinant Antibody (D1664) (CBMAB-D1664-YC)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
D1664
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Full length of human DR1.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DR1
Introduction
DR1 is a TBP- (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The encoded protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription.
Entrez Gene ID
Human1810
Mouse13486
Rat289881
UniProt ID
HumanQ01658
MouseQ91WV0
RatQ5XI68
Alternative Names
Down-Regulator Of Transcription 1; TATA-Binding Protein-Associated Phosphoprotein; Negative Cofactor 2-Beta; Negative Cofactor 2; NC2-BETA; Down-Regulator Of Transcription 1, TBP-Binding (Negative Cofactor 2);
Function
The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.
Biological Process
Chromatin remodeling Source: GO_Central
Histone H3 acetylation Source: BHF-UCL
Negative regulation of transcription by RNA polymerase II Source: ARUK-UCL
Positive regulation of transcription by RNA polymerase II Source: GO_Central
RNA polymerase II preinitiation complex assembly Source: GO_Central
Cellular Location
Nucleus
PTM
Phosphorylation regulates its interaction with TBP. Not phosphorylated when bound to DRAP1.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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