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Mouse Anti-DSTYK Recombinant Antibody (CBCND-021) (V2LY-0425-LY1021)

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Published Data
Mouse Anti-DSTYK Recombinant Antibody (CBCND-021) (V2LY-0425-LY1021-1) in Immunoblot
Mouse Anti-DSTYK Recombinant Antibody (CBCND-021) (V2LY-0425-LY1021-1) in Immunoblot
Immunoblotting of endogenous TFEB, p-TFEB (phosphorylated TFEB) and p-S6K(T389) in COS-7 cells transfected with control siRNA (siCtrl) or Dstyk siRNA (siDs). ...View More
Sun, X., Zhou, Y., Zhang, R., Wang, Z., Xu, M., Zhang, D., ... & Chen, L. (2020). Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway. Nature Communications, 11(1), 479.
Mouse Anti-DSTYK Recombinant Antibody (CBCND-021) (V2LY-0425-LY1021-2) in IF
Mouse Anti-DSTYK Recombinant Antibody (CBCND-021) (V2LY-0425-LY1021-2) in IF
Immunofluorescence for TFEB and LAMP1 in COS-7 cells transfected with control siRNA or Dstyk siRNA, cells without starvation (Control) or starved for 2h. ...View More
Sun, X., Zhou, Y., Zhang, R., Wang, Z., Xu, M., Zhang, D., ... & Chen, L. (2020). Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway. Nature Communications, 11(1), 479.
Mouse Anti-DSTYK Recombinant Antibody (CBCND-021) (V2LY-0425-LY1021-3) in IF
Mouse Anti-DSTYK Recombinant Antibody (CBCND-021) (V2LY-0425-LY1021-3) in IF
Immunofluorescence microscopy using a DSTYK C-terminal antibody in normal skin (top) reveals bright pan-epidermal cytoplasmic staining with focal accentuation in basal epidermal melanocytes and dermal fibroblasts. In affected individual (IV-5) skin (middle) there is markedly reduced labeling, with an absence of staining in the negative control (bottom). ...View More
Lee, J. Y., Hsu, C. K., Michael, M., Nanda, A., Liu, L., McMillan, J. R., ... & McGrath, J. A. (2017). Large intragenic deletion in DSTYK underlies autosomal-recessive complicated spastic paraparesis, SPG23. The American Journal of Human Genetics, 100(2), 364-370.
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Basic Information

Host Animal
Mouse
Clone
CBCND-021
Application
WB, IP, IF, ELISA, IHC-P
Immunogen
Amino acids 630-929 mapping at the C-terminus of human RIP5.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
dual serine/threonine and tyrosine protein kinase
Entrez Gene ID
25778
UniProt ID
Q6XUX3
Research Area
Acts as a positive regulator of ERK phosphorylation downstream of fibroblast growth factor-receptor activation (PubMed:23862974, PubMed:28157540).

Involved in the regulation of both caspase-dependent apoptosis and caspase-independent cell death (PubMed:15178406).

In the skin, it plays a predominant role in suppressing caspase-dependent apoptosis in response to UV stress in a range of dermal cell types (PubMed:28157540).
Biological Process
Cellular response to fibroblast growth factor stimulus Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Positive regulation of fibroblast growth factor receptor signaling pathway Source: UniProtKB
Positive regulation of kinase activity Source: UniProtKB
Cellular Location
Cell membrane; Apical cell membrane; Basolateral cell membrane; Cytoplasm; Cell junction. Detected at apical cell-cell junctions. Colocalized with FGF receptors to the cell membrane (By similarity). Detected in basolateral and apical membranes of all tubular epithelia.
Involvement in disease
Congenital anomalies of the kidney and urinary tract 1 (CAKUT1):
A disorder encompassing a broad spectrum of renal and urinary tract malformations that include renal agenesis, kidney hypodysplasia, multicystic kidney dysplasia, duplex collecting system, posterior urethral valves and ureter abnormalities. Congenital anomalies of kidney and urinary tract are the commonest cause of chronic kidney disease in children.
Spastic paraplegia 23, autosomal recessive (SPG23):
A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SPG23 is an autosomal recessive form characterized by childhood-onset of gait difficulties and pigmentary abnormalities, including premature graying of the hair and vitiligo-like or hyperpigmented skin lesions.

Zhong, C., Chen, M., Chen, Y., Yao, F., & Fang, W. (2021). Loss of DSTYK activates Wnt/β-catenin signaling and glycolysis in lung adenocarcinoma. Cell death & disease, 12(12), 1-10.

Ogbu, S. C., Rojas, S., Weaver, J., Musich, P. R., Zhang, J., Yao, Z. Q., & Jiang, Y. (2021). DSTYK Enhances Chemoresistance in Triple-Negative Breast Cancer Cells. Cells, 11(1), 97.

Vidic, C., Zaniew, M., Jurga, S., Thiele, H., Reutter, H., & Hilger, A. C. (2021). Exome sequencing implicates a novel heterozygous missense variant in DSTYK in autosomal dominant lower urinary tract dysfunction and mild hereditary spastic paraparesis. Molecular and cellular pediatrics, 8(1), 1-6.

Sun, X., Zhou, Y., Zhang, R., Wang, Z., Xu, M., Zhang, D., ... & Chen, L. (2020). Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway. Nature communications, 11(1), 1-17.

Zhang, J., Miller, Z., Musich, P. R., Thomas, A. E., Yao, Z. Q., Xie, Q., ... & Jiang, Y. (2020). DSTYK promotes metastasis and chemoresistance via EMT in colorectal cancer. Frontiers in pharmacology, 1250.

Tang, G., Yang, Y., Shang, L., Jun, F., & Liu, Q. (2019). A DSTYK mutation activates ERK1/2 signaling to promote intraspinal dissemination in a case of solitary fibrous tumor/hemangiopericytoma. Laboratory Investigation, 99(10), 1501-1514.

Lee, J. Y., Hsu, C. K., Michael, M., Nanda, A., Liu, L., McMillan, J. R., ... & McGrath, J. A. (2017). Large intragenic deletion in DSTYK underlies autosomal-recessive complicated spastic paraparesis, SPG23. The American Journal of Human Genetics, 100(2), 364-370.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-DSTYK Recombinant Antibody (CBCND-021) (CAT#: CBMAB-R0509-CN)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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