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Mouse Anti-DSTYK Recombinant Antibody (4F3) (CBMAB-D1828-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all DSTYK antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4F3
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
DSTYK (NP_056190, 80 a.a. ~ 189 a.a) partial recombinant protein with GST tag. The immunogen sequence: DVAETGLQAG QLSCISFPPK EEKYLQQIVD CLPCILILGQ DCNVKCQLLN LLLGVQVLPT TKLGSEESCK LRRLRFTYGT QTRVSLALPG QYELVHTLVA HQGNWETIPE
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 80-189

Target

Full Name
dual serine/threonine and tyrosine protein kinase
Introduction
DSTYK is a dual serine/threonine and tyrosine protein kinase which is expressed in multiple tissues. It is thought to function as a regulator of cell death.
Entrez Gene ID
25778
UniProt ID
Q6XUX3
Alternative Names
Dual Serine/Threonine And Tyrosine Protein Kinase; Receptor-Interacting Serine/Threonine-Protein Kinase 5; RIP-Homologous Kinase; Dusty Protein Kinase; Sugen Kinase 496; EC 2.7.12.1; Dusty PK; SgK496; RIPK5;
Research Area
Acts as a positive regulator of ERK phosphorylation downstream of fibroblast growth factor-receptor activation (PubMed:23862974, PubMed:28157540).

Involved in the regulation of both caspase-dependent apoptosis and caspase-independent cell death (PubMed:15178406).

In the skin, it plays a predominant role in suppressing caspase-dependent apoptosis in response to UV stress in a range of dermal cell types (PubMed:28157540).
Biological Process
Cellular response to fibroblast growth factor stimulus Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Positive regulation of fibroblast growth factor receptor signaling pathway Source: UniProtKB
Positive regulation of kinase activity Source: UniProtKB
Cellular Location
Cell membrane; Apical cell membrane; Basolateral cell membrane; Cytoplasm; Cell junction. Detected at apical cell-cell junctions. Colocalized with FGF receptors to the cell membrane (By similarity). Detected in basolateral and apical membranes of all tubular epithelia.
Involvement in disease
Congenital anomalies of the kidney and urinary tract 1 (CAKUT1):
A disorder encompassing a broad spectrum of renal and urinary tract malformations that include renal agenesis, kidney hypodysplasia, multicystic kidney dysplasia, duplex collecting system, posterior urethral valves and ureter abnormalities. Congenital anomalies of kidney and urinary tract are the commonest cause of chronic kidney disease in children.
Spastic paraplegia 23, autosomal recessive (SPG23):
A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SPG23 is an autosomal recessive form characterized by childhood-onset of gait difficulties and pigmentary abnormalities, including premature graying of the hair and vitiligo-like or hyperpigmented skin lesions.

Zhong, C., Chen, M., Chen, Y., Yao, F., & Fang, W. (2021). Loss of DSTYK activates Wnt/β-catenin signaling and glycolysis in lung adenocarcinoma. Cell death & disease, 12(12), 1-10.

Ogbu, S. C., Rojas, S., Weaver, J., Musich, P. R., Zhang, J., Yao, Z. Q., & Jiang, Y. (2021). DSTYK Enhances Chemoresistance in Triple-Negative Breast Cancer Cells. Cells, 11(1), 97.

Vidic, C., Zaniew, M., Jurga, S., Thiele, H., Reutter, H., & Hilger, A. C. (2021). Exome sequencing implicates a novel heterozygous missense variant in DSTYK in autosomal dominant lower urinary tract dysfunction and mild hereditary spastic paraparesis. Molecular and cellular pediatrics, 8(1), 1-6.

Sun, X., Zhou, Y., Zhang, R., Wang, Z., Xu, M., Zhang, D., ... & Chen, L. (2020). Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway. Nature communications, 11(1), 1-17.

Zhang, J., Miller, Z., Musich, P. R., Thomas, A. E., Yao, Z. Q., Xie, Q., ... & Jiang, Y. (2020). DSTYK promotes metastasis and chemoresistance via EMT in colorectal cancer. Frontiers in pharmacology, 1250.

Tang, G., Yang, Y., Shang, L., Jun, F., & Liu, Q. (2019). A DSTYK mutation activates ERK1/2 signaling to promote intraspinal dissemination in a case of solitary fibrous tumor/hemangiopericytoma. Laboratory Investigation, 99(10), 1501-1514.

Lee, J. Y., Hsu, C. K., Michael, M., Nanda, A., Liu, L., McMillan, J. R., ... & McGrath, J. A. (2017). Large intragenic deletion in DSTYK underlies autosomal-recessive complicated spastic paraparesis, SPG23. The American Journal of Human Genetics, 100(2), 364-370.

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For research use only. Not intended for any clinical use.

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