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Mouse Anti-DUSP12 Recombinant Antibody (AF28B2) (CBMAB-D1881-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Dual Specificity Phosphatase 12 (DUSP12). The antibody can be used for immunoassay techniques, such as WB, ELISA.
See all DUSP12 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
AF28B2
Antibody Isotype
IgG1
Application
WB, ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Dual Specificity Phosphatase 12
Introduction
DUSP12 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product is the human ortholog of the Saccharomyces cerevisiae YVH1 protein tyrosine phosphatase. It is localized predominantly in the nucleus, and is novel in that it contains, and is regulated by a zinc finger domain.
Entrez Gene ID
11266
UniProt ID
Q9UNI6
Alternative Names
Dual Specificity Phosphatase 12; Serine/Threonine Specific Protein Phosphatase; Dual Specificity Tyrosine Phosphatase YVH1; YVH1 Protein-Tyrosine Phosphatase (S. Cerevisiae) Ortholog; YVH1 Protein-Tyrosine Phosphatase Ortholog; Dual Specificity Protein Phosphatase 12;
Research Area
Dual specificity phosphatase; can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues. Can dephosphorylate glucokinase (in vitro) (By similarity).

Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate and other in vitro substrates (PubMed:10446167, PubMed:24531476).
Biological Process
Cellular protein modification process Source: ProtInc
Dephosphorylation Source: UniProtKB
Positive regulation of glucokinase activity Source: GO_Central
Protein dephosphorylation Source: GO_Central
Cellular Location
Nucleus; Cytosol. Primarily nuclear. Detected in a mesh-like pattern in the cytosol.

Boldorini, R., Clemente, N., Alchera, E., & Carini, R. (2021). DUSP12 acts as a novel endogenous protective signal against hepatic ischemia–reperfusion damage by inhibiting ASK1 pathway. Clinical Science, 135(1), 161-166.

Ju, G., Zhou, T., Zhang, R., Pan, X., Xue, B., & Miao, S. (2021). DUSP12 regulates the tumorigenesis and prognosis of hepatocellular carcinoma. PeerJ, 9, e11929.

Qiu, T., Wang, T., Zhou, J., Chen, Z., Zou, J., Zhang, L., & Ma, X. (2020). DUSP12 protects against hepatic ischemia–reperfusion injury dependent on ASK1-JNK/p38 pathway in vitro and in vivo. Clinical Science, 134(17), 2279-2294.

Huang, Z., Wu, L. M., Zhang, J. L., Sabri, A., Wang, S. J., Qin, G. J., ... & Zhang, Y. Z. (2019). Dual specificity phosphatase 12 regulates hepatic lipid metabolism through inhibition of the lipogenesis and apoptosis signal–regulating kinase 1 pathways. Hepatology, 70(4), 1099-1118.

Monteiro, L. F., & Forti, F. L. (2019). Network analysis of DUSP12 partners in the nucleus under genotoxic stress. Journal of proteomics, 197, 42-52.

Cho, S. S. L., Han, J., James, S. J., Png, C. W., Weerasooriya, M., Alonso, S., & Zhang, Y. (2017). Dual-specificity phosphatase 12 targets p38 MAP kinase to regulate macrophage response to intracellular bacterial infection. Frontiers in immunology, 8, 1259.

Li, W. M., Zhao, Y. F., Zhu, G. F., Peng, W. H., Zhu, M. Y., Yu, X. J., ... & Xu, Y. W. (2017). Dual specific phosphatase 12 ameliorates cardiac hypertrophy in response to pressure overload. Clinical Science, 131(2), 141-154.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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