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Mouse Anti-DUSP12 Recombinant Antibody (D1881) (V2LY-0425-LY1058)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
D1881
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 254-340 mapping at the C-terminus of human DUSP12.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Dual Specificity Phosphatase 12
Entrez Gene ID
11266
UniProt ID
Q9UNI6
Research Area
Dual specificity phosphatase; can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues. Can dephosphorylate glucokinase (in vitro) (By similarity).

Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate and other in vitro substrates (PubMed:10446167, PubMed:24531476).
Biological Process
Cellular protein modification process Source: ProtInc
Dephosphorylation Source: UniProtKB
Positive regulation of glucokinase activity Source: GO_Central
Protein dephosphorylation Source: GO_Central
Cellular Location
Nucleus; Cytosol. Primarily nuclear. Detected in a mesh-like pattern in the cytosol.
More Infomation

Boldorini, R., Clemente, N., Alchera, E., & Carini, R. (2021). DUSP12 acts as a novel endogenous protective signal against hepatic ischemia–reperfusion damage by inhibiting ASK1 pathway. Clinical Science, 135(1), 161-166.

Ju, G., Zhou, T., Zhang, R., Pan, X., Xue, B., & Miao, S. (2021). DUSP12 regulates the tumorigenesis and prognosis of hepatocellular carcinoma. PeerJ, 9, e11929.

Qiu, T., Wang, T., Zhou, J., Chen, Z., Zou, J., Zhang, L., & Ma, X. (2020). DUSP12 protects against hepatic ischemia–reperfusion injury dependent on ASK1-JNK/p38 pathway in vitro and in vivo. Clinical Science, 134(17), 2279-2294.

Huang, Z., Wu, L. M., Zhang, J. L., Sabri, A., Wang, S. J., Qin, G. J., ... & Zhang, Y. Z. (2019). Dual specificity phosphatase 12 regulates hepatic lipid metabolism through inhibition of the lipogenesis and apoptosis signal–regulating kinase 1 pathways. Hepatology, 70(4), 1099-1118.

Monteiro, L. F., & Forti, F. L. (2019). Network analysis of DUSP12 partners in the nucleus under genotoxic stress. Journal of proteomics, 197, 42-52.

Cho, S. S. L., Han, J., James, S. J., Png, C. W., Weerasooriya, M., Alonso, S., & Zhang, Y. (2017). Dual-specificity phosphatase 12 targets p38 MAP kinase to regulate macrophage response to intracellular bacterial infection. Frontiers in immunology, 8, 1259.

Li, W. M., Zhao, Y. F., Zhu, G. F., Peng, W. H., Zhu, M. Y., Yu, X. J., ... & Xu, Y. W. (2017). Dual specific phosphatase 12 ameliorates cardiac hypertrophy in response to pressure overload. Clinical Science, 131(2), 141-154.

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For research use only. Not intended for any clinical use.

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