Mouse Anti-DUSP27 Recombinant Antibody (AF16B7) (CBMAB-D1892-YC)

Name: Mouse Anti-DUSP27 Recombinant Antibody (AF16B7)
SKU: CBMAB-D1892-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

AF16B7

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 60-86 within an internal region of human DUSP27.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgM, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Dual Specificity Phosphatase 27, Atypical

Introduction

DUSP27 (Dual Specificity Phosphatase 27, Atypical) is a protein coding gene. It has proved that the protein has phosphatase activity and protein tyrosine/serine/threonine phosphatase activity. And a paralog is DUPD1.

Entrez Gene ID

92235

UniProt ID

Q5VZP5

Alternative Names

Dual Specificity Phosphatase 27, Atypical; Dual Specificity Phosphatase 27 (Putative);

Research Area

Dual specificity phosphatase able to dephosphorylate phosphotyrosine, phosphoserine and phosphothreonine residues within the same substrate, with a preference for phosphotyrosine as a substrate (PubMed:17498703).

Involved in the modulation of intracellular signaling cascades. In skeletal muscle regulates systemic glucose homeostasis by activating, AMPK, an energy sensor protein kinase (By similarity).

Affects MAP kinase signaling though modulation of the MAPK1/2 cascade in skeletal muscle promoting muscle cell differentiation, development and atrophy (By similarity).

Biological Process

Glucose homeostasis Source: Ensembl
Muscle cell differentiation Source: UniProtKB
Negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
Protein dephosphorylation Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm

Cooper, L. M., & Waddell, D. S. (2020). A tale of two DUSP27s: proposed resolution for the naming of distinct dual-specificity phosphatases. American Journal of Physiology-Cell Physiology, 319(1), C148-C150.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-DUSP27 Recombinant Antibody (1F3) (CAT#: CBMAB-D1891-YC)

Isotype control

For research use only. Not intended for any clinical use.

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