Mouse Anti-DUSP5 Recombinant Antibody (2F3) (CBMAB-D1911-YC)

Name: Mouse Anti-DUSP5 Recombinant Antibody (2F3)
SKU: CBMAB-D1911-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DUSP5 Recombinant Antibody (2F3) (CBMAB-D1911-YC-1) in WB

Comparison of the expression and activity of Dusp5 in WBCs isolated from Dusp5 ZFN KO versus FHH.1BN rats.
Panel A: The expression of Dusp5 protein in WBCs is nearly absent in Dusp5 KO versus FHH.1BN rats. The levels of phosphorylated-ERK1/2 protein are significantly increased in Dusp5 KO compared to FHH.1BN rats, but there is no change in the expression of total ERK or beta-Actin. Panel B presents the results of the expression of Dusp5, p-ERK1/2, total ERK1/2 and β-Actin protein from duplicate aliquots of a single pooled microvessel sample isolated from 8-10 Dusp5 KO and FHH.1BN rats. The upper panel presents a representative image of the gels and the lower panel presents the quantitation of the images above. Mean values ± SE from 3 rats per strain are presented in Panel A. ...View More

Fan, F., Geurts, A. M., Pabbidi, M. R., Smith, S. V., Harder, D. R., Jacob, H., & Roman, R. J. (2014). Zinc-finger nuclease knockout of dual-specificity protein phosphatase-5 enhances the myogenic response and autoregulation of cerebral blood flow in FHH. 1BN rats. PloS one, 9(11), e112878.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2F3

Application

IF, WB, ELISA

Immunogen

DUSP5 (aa286-385) partial recombinant protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 286-384

More Infomation

Target

Full Name

dual specificity phosphatase 5

Introduction

DUSP5 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus.

Entrez Gene ID

1847

UniProt ID

Q16690

Alternative Names

Dual Specificity Phosphatase 5; Dual Specificity Protein Phosphatase HVH3; Serine/Threonine Specific Protein Phosphatase; Dual Specificity Protein Phosphatase 5; VH1-Like Phosphatase 3; EC 3.1.3.16;

Research Area

Dual specificity protein phosphatase; active with phosphotyrosine, phosphoserine and phosphothreonine residues. The highest relative activity is toward ERK1.

Biological Process

Activation of MAPK activity Source: Reactome
Dephosphorylation Source: UniProtKB
Endoderm formation Source: GO_Central
Inactivation of MAPK activity Source: GO_Central
MAPK cascade Source: Reactome
Peptidyl-threonine dephosphorylation Source: UniProtKB
Peptidyl-tyrosine dephosphorylation Source: UniProtKB
Protein dephosphorylation Source: GO_Central

Cellular Location

Nucleus

Hua, C., Zheng, Q., Zhu, J., Chen, S., Song, Y., van der Veen, S., & Cheng, H. (2022). Human Papillomavirus Type 16 Early Protein E7 Activates Autophagy through Inhibition of Dual-Specificity Phosphatase 5. Oxidative medicine and cellular longevity, 2022.

Kidger, A. M., Saville, M. K., Rushworth, L. K., Davidson, J., Stellzig, J., Ono, M., ... & Keyse, S. M. (2022). Suppression of mutant Kirsten-RAS (KRASG12D)-driven pancreatic carcinogenesis by dual-specificity MAP kinase phosphatases 5 and 6. Oncogene, 41(20), 2811-2823.

Tan, L., Bogush, N., Naqvi, E., Calvert, J. W., Graham, R. M., Taylor, W. R., ... & Husain, A. (2021). Thyroid hormone plus dual-specificity phosphatase-5 siRNA increases the number of cardiac muscle cells and improves left ventricular contractile function in chronic doxorubicin-injured hearts. Theranostics, 11(10), 4790.

Luo, J., Xue, D., Song, F., Liu, X., Li, W., & Wang, Y. (2020). DUSP5 (dual-specificity protein phosphatase 5) suppresses BCG-induced autophagy via ERK 1/2 signaling pathway. Molecular Immunology, 126, 101-109.

Zhang, H., Zhang, C., Liu, Y., Gao, W., Wang, S., Fang, X., ... & Fan, F. (2020). Influence of dual‐specificity protein phosphatase 5 on mechanical properties of rat cerebral and renal arterioles. Physiological Reports, 8(2), e14345.

Zhang, C., He, X., Murphy, S. R., Zhang, H., Wang, S., Ge, Y., ... & Fan, F. (2019). Knockout of dual-specificity protein phosphatase 5 protects against hypertension-induced renal injury. Journal of Pharmacology and Experimental Therapeutics, 370(2), 206-217.

Wang, L., Hu, J., Qiu, D., Gao, H., Zhao, W., Huang, Y., ... & Chen, Y. (2019). Dual-specificity phosphatase 5 suppresses ovarian cancer progression by inhibiting IL-33 signaling. American Journal of Translational Research, 11(2), 844.

Alleboina, S., Ayalew, D., Peravali, R., Chen, L., Wong, T., & Dokun, A. O. (2019). Dual specificity phosphatase 5 regulates perfusion recovery in experimental peripheral artery disease. Vascular Medicine, 24(5), 395-404.

Gupta, A., Brahmbhatt, J., Syrlybaeva, R., Bodnar, C., Bodnar, N., Bongard, R., ... & Talipov, M. R. (2019). Role of conserved histidine and serine in the HCXXXXXRS motif of human dual-specificity phosphatase 5. Journal of Chemical Information and Modeling, 59(4), 1563-1574.

Zhang, C., He, X., Wang, S., Roman, R. J., Booz, G., & Fan, F. (2018). Knockout of Dual-Specificity Protein Phosphatase 5 Protects Against Hypertension Induced Chronic Kidney Disease. Hypertension, 72(Suppl_1), A056-A056.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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