Mouse Anti-DZIP3 Recombinant Antibody (D2037) (CBMAB-D2037-YC)

Name: Mouse Anti-DZIP3 Recombinant Antibody (D2037)
SKU: CBMAB-D2037-YC
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

D2037

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 47-302 mapping near the N-terminus of human DZIP3.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

DAZ Interacting Zinc Finger Protein 3

Introduction

DZIP3 (DAZ Interacting Zinc Finger Protein 3) is a protein coding gene. Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation. It has proved that the protein has ligase activity. And a paralog is TTC3.

Entrez Gene ID

9666

UniProt ID

Q86Y13

Alternative Names

DAZ Interacting Zinc Finger Protein 3; Human RNA-Binding Ubiquitin Ligase Of 138 KDa; Protein Phosphatase 1, Regulatory Subunit 66; RING-Type E3 Ubiquitin Transferase DZIP3; RNA-Binding Ubiquitin Ligase Of 138 KDa; DAZ Interacting Protein 3, Zinc Finger; DAZ-Interacting Protein 3; HRUL138; RNA-Binding RING-H2 Protein-Ubiquitin Ligase;

Research Area

E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.

Biological Process

Protein polyubiquitination Source: UniProtKB

Cellular Location

Cytoplasm

More Infomation

References

Kolapalli, S. P., Sahu, R., Chauhan, N. R., Jena, K. K., Mehto, S., Das, S. K., ... & Chauhan, S. (2021). RNA-Binding RING E3-Ligase DZIP3/hRUL138 Stabilizes Cyclin D1 to Drive Cell-Cycle and Cancer ProgressionDZIP3 Is a Novel Driver of Cell-Cycle and Cancer Progression. Cancer research, 81(2), 315-331.

Mamoor, S. (2021). DZIP3 is a differentially expressed gene in human metastatic breast cancer, in the brain and in the lymph nodes.

Quan, Y., Liang, F., Wu, D., Yao, X., Hu, Z., Zhu, Y., ... & Xiong, J. (2020). Blood cell DNA methylation of aging-related ubiquitination gene DZIP3 can predict the onset of early stage colorectal cancer. Frontiers in Oncology, 10, 544330.

Liang, T., Zhou, X., Li, P., You, G., Wang, F., Wang, P., & Feng, E. (2020). DZIP3 is a key factor to stratify IDH1 wild-type lower-grade gliomas. Aging (Albany NY), 12(24), 24995.

Oikawa, D., Shiota, M., Tokunaga, F., & Wanibuchi, H. (2018). Generation of rat monoclonal antibodies specific for DZIP3. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 37(3), 153-157.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-DZIP3 Recombinant Antibody (3A1)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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