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Mouse Anti-E2F4 Recombinant Antibody (CBFYE-0371) (CBMAB-E0672-FY)

This product is mouse antibody that recognizes E2F4. The antibody CBFYE-0371 can be used for immunoassay techniques such as: ELISA, IP, WB.
See all E2F4 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYE-0371
Antibody Isotype
IgG2b
Application
ELISA, IP, WB

Basic Information

Immunogen
Fusion Protein corresponding to the human E2F-4 protein
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1M Tris-glycine buffer, pH 7.4, 0.15M sodium chloride, 30%glycerol
Preservative
0.05% Sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
E2F4
Introduction
This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.
Entrez Gene ID
Human1874
Mouse104394
Rat100360427
UniProt ID
HumanQ16254
MouseQ8R0K9
RatD4A9V4
Alternative Names
E2F-4
Research Area
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
Biological Process
Animal organ morphogenesis Source: Ensembl
Blood circulation Source: Ensembl
Cell volume homeostasis Source: Ensembl
Centriole assembly Source: UniProtKB
Epithelial cell development Source: Ensembl
Motile cilium assembly Source: UniProtKB
Multi-ciliated epithelial cell differentiation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of cell population proliferation Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Ensembl
Cellular Location
Nucleus
PTM
Differentially phosphorylated in vivo.

Dong, C., Yue, X., Zhu, L., Ge, P., Zheng, G., Ye, Z., & Pan, B. (2022). Curcumin Attenuates Hydrocephalus via Activation of E2F Transcription Factor 4. Cerebrovascular Diseases, 1-8.

Xiao, W., Wang, J., Wang, X., Cai, S., Guo, Y., Ye, L., ... & Zheng, L. (2022). Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression. Autophagy, 1-21.

Chen, P., Wang, L., Long, Y. B., Liang, G. Y., Yang, X., Dong, Z. Q., ... & Lu, C. (2022). E2F4 regulates the cell cycle and DNA replication in the silkworm, Bombyx mori. Insect Science, 29(4), 1006-1016.

Wei, J., Shi, Y., Zou, C., Zhang, H., Peng, H., Wang, S., ... & Wang, D. (2022). Cellular Id1 inhibits hepatitis B virus transcription by interacting with the novel covalently closed circular DNA-binding protein E2F4. International journal of biological sciences, 18(1), 65.

Zheng, Q., Fu, Q., Xu, J., Gu, X., Zhou, H., & Zhi, C. (2021). Transcription factor E2F4 is an indicator of poor prognosis and is related to immune infiltration in hepatocellular carcinoma. Journal of Cancer, 12(6), 1792.

Liu, J., Xia, L., Wang, S., Cai, X., Wu, X., Zou, C., ... & Wang, D. (2021). E2F4 Promotes the Proliferation of Hepatocellular Carcinoma Cells through Upregulation of CDCA3. Journal of Cancer, 12(17), 5173.

Kim, J., Yim, G. W., Lee, D. W., Kim, Y. T., Lee, Y. J., & Rhee, Y. J. (2021). Knockdown of E2F4 suppresses the growth of ovarian cancer cells through the cell cycle pathway. International Journal of Clinical and Experimental Pathology, 14(8), 866.

Hong, J., Wang, X., Mei, C., & Zan, L. (2019). Competitive regulation by transcription factors and DNA methylation in the bovine SIRT5 promoter: Roles of E2F4 and KLF6. Gene, 684, 39-46.

Hsu, J., Arand, J., Chaikovsky, A., Mooney, N. A., Demeter, J., Brison, C. M., ... & Sage, J. (2019). E2F4 regulates transcriptional activation in mouse embryonic stem cells independently of the RB family. Nature communications, 10(1), 1-14.

Bhawe, K., & Roy, D. (2018). Interplay between NRF1, E2F4 and MYC transcription factors regulating common target genes contributes to cancer development and progression. Cellular Oncology, 41(5), 465-484.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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