Mouse Anti-E2F5 Recombinant Antibody (E0678) (CBMAB-E0678-FY)

Name: Mouse Anti-E2F5 Recombinant Antibody (E0678)
SKU: CBMAB-E0678-FY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat, Horse, Dog, Cattle, Pig

Clone

E0678

Antibody Isotype

IgG2a, κ

Application

WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen

Amino acids 303-331 at the C-terminus of human E2F-5.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Horse, Dog, Cattle, Pig

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

E2F transcription factor 5

Introduction

This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms.

Entrez Gene ID

Human	1875
Mouse	13559
Rat	116651

UniProt ID

Human	Q15329
Mouse	Q61502
Rat	Q62814

Alternative Names

E2F-5

Research Area

Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.

Biological Process

Cell projection organization Source: UniProtKB-KW
Regulation of cell cycle Source: InterPro
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus

More Infomation

References

Fan, X., Liu, M., Fei, L., Huang, Z., & Yan, Y. (2022). CircFOXM1 promotes the proliferation, migration, invasion, and glutaminolysis of glioblastoma by regulating the miR-577/E2F transcription factor 5 axis. Bosnian Journal of Basic Medical Sciences, 22(2), 205.

Wang, Y., Wang, X., Han, L., & Hu, D. (2020). LncRNA MALAT1 regulates the progression and cisplatin resistance of ovarian cancer cells via modulating miR-1271-5p/E2F5 axis. Cancer Management and Research, 12, 9999.

Inagaki, Y., Wu, D., Fujiwara, K., Ishizuka, Y., Oguni, A., Tokunaga, T., ... & Masuda, S. (2020). Knockdown of E2F5 induces cell death via the TP53‑dependent pathway in breast cancer cells carrying wild‑type TP53. Oncology Reports, 44(5), 2241-2252.

Sun, F., Zhang, C., Ma, D., & Wang, K. (2019). MicroRNA‑544 inhibits esophageal squamous cell carcinoma cell proliferation and enhances sensitivity to cisplatin by repressing E2F transcription factor 5. Oncology Letters, 18(4), 4203-4209.

Yang, F., Chen, L., & Wang, Z. J. (2019). MicroRNA-32 inhibits the proliferation, migration and invasion of human colon cancer cell lines by targeting E2F transcription factor 5. Eur Rev Med Pharmacol Sci, 23(10), 4156-4163.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-E2F5 Recombinant Antibody (CBFYE-0376)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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