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Mouse Anti-E2F5 Recombinant Antibody (220D1a) (CBMAB-E0678-FY)

This product is mouse antibody that recognizes E2F5. The antibody 220D1a can be used for immunoassay techniques such as: DB, ICC, IF, WB.
See all E2F5 antibodies
Published Data
Fig.1 Western blot analysis showing differences in the expression levels of let-7e and E2F5.
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Fig.2 Expression pattern of E2F5 in primary hepatocellular carcinoma by tissue microarray based immunohistochemistry.
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Fig.3 Cells were immunostained with antibody against E2F5, or Flag (NS1), followed by secondary antibody conjugated with FITC (green) for individual E2Fs or with Alexa Fluor 568 (red) for Flag (NS1). After counterstaining of nuclei with DAPI (blue), cells were examined by confocal microscopy.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
220D1a
Antibody Isotype
IgG1
Application
DB, ICC, IF, WB

Basic Information

Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2, 1% BSA
Preservative
0.05% Sodium azide
Concentration
0.1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
E2F transcription factor 5
Introduction
This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms.
Entrez Gene ID
1875
UniProt ID
Q15329
Alternative Names
E2F-5
Research Area
Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
Biological Process
Cell projection organization Source: UniProtKB-KW
Regulation of cell cycle Source: InterPro
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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