Rabbit Anti-E2F6 Recombinant Antibody (E0682) (V2LY-0425-LY1251)

Name: Rabbit Anti-E2F6 Recombinant Antibody (E0682)
SKU: V2LY-0425-LY1251
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse

Clone

E0682

Antibody Isotype

IgG

Application

WB, IHC

Basic Information

Immunogen

Recombinant protein within Human E2F6 (aa 182-281).

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000
IHC	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & Glycerol & TBS

Preservative

Sodium Azide

Concentration

1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

E2F Transcription Factor 6

Entrez Gene ID

Human	1876
Mouse	50496

UniProt ID

Human	O75461
Mouse	O54917

Research Area

Inhibitor of E2F-dependent transcription (PubMed:9689056, PubMed:9704927, PubMed:9501179).

Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' (PubMed:9501179).

Has a preference for the 5'-TTTCCCGC-3' E2F recognition site (PubMed:9501179).

E2F6 lacks the transcriptional activation and pocket protein binding domains (PubMed:9704927, PubMed:9501179).

Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression (PubMed:9689056, PubMed:9501179).

Represses expression of some meiosis-specific genes, including SLC25A31/ANT4 (By similarity).

May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase (PubMed:9501179).

Biological Process

Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Ensembl

Cellular Location

Nucleus

More Infomation

References

Liu, Y., Yao, Y., Liao, B., Zhang, H., Yang, Z., Xia, P., ... & Yuan, Y. (2022). A positive feedback loop of CENPU/E2F6/E2F1 facilitates proliferation and metastasis via ubiquitination of E2F6 in hepatocellular carcinoma. International journal of biological sciences, 18(10), 4071-4087.

Chen, G., Wang, K., Li, G., Wang, L., Xiao, Y., & Chen, B. (2022). Long noncoding RNA LAMTOR5-AS1 interference affects microRNA-506-3p/E2F6-mediated behavior of non-small cell lung cancer cells. Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics, 28(9), 945-959.

Gu, Q., Hou, W., Shi, L., Liu, H., Zhu, Z., & Ye, W. (2021). Circular RNA ZNF609 functions as a competing endogenous RNA in regulating E2F transcription factor 6 through competitively binding to microRNA-197-3p to promote the progression of cervical cancer progression. Bioengineered, 12(1), 927-936.

Li, J., Li, D., Zhang, X., Li, C., & Zhu, F. (2021). Long noncoding RNA SLC9A3‑AS1 increases E2F6 expression by sponging microRNA‑486‑5p and thus facilitates the oncogenesis of nasopharyngeal carcinoma. Oncology Reports, 46(2), 1-13.

LAFTA, I. (2019). E2F6 is essential for cell viability in breast cancer cells during replication stress. Turkish Journal of Biology, 43(5), 293-304.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Antibody Pairs

E2F6 Matched Antibody Pair (357)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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