Rabbit Anti-E2F6 Recombinant Antibody (E0682) (V2LY-0425-LY1251)
Basic Information
| Application | Note |
| WB | 1:1,000 |
| IHC | 1:1,000 |
Formulations & Storage [For reference only, actual COA shall prevail!]
Target
Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' (PubMed:9501179).
Has a preference for the 5'-TTTCCCGC-3' E2F recognition site (PubMed:9501179).
E2F6 lacks the transcriptional activation and pocket protein binding domains (PubMed:9704927, PubMed:9501179).
Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression (PubMed:9689056, PubMed:9501179).
Represses expression of some meiosis-specific genes, including SLC25A31/ANT4 (By similarity).
May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase (PubMed:9501179).
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Ensembl
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
Antibody Pairs
E2F6 Matched Antibody Pair (357) (CAT#: APMAB-357LY)
Related Products
Mouse Anti-E2F6 Monoclonal Antibody (1F6) (CAT#: CBMAB-0324-YC)
Mouse Anti-E2F6 Recombinant Antibody (XB0371) (CAT#: V2LY-0425-LY1255)
Mouse Anti-E2F6 Recombinant Antibody (CBFYE-0378) (CAT#: V2LY-0425-LY1253)
Mouse Anti-E2F6 Recombinant Antibody (2B6-G9) (CAT#: CBMAB-A2418-LY)
E2F6 Matched Antibody Pair (357) (CAT#: APMAB-357LY)
Mouse Anti-E2F6 Recombinant Antibody (2B6-G9) (CAT#: V2LY-0425-LY1250)
Mouse Anti-E2F6 Recombinant Antibody (CBFYE-0379) (CAT#: V2LY-0425-LY1254)
Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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