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Mouse Anti-E2F6 Recombinant Antibody (XB0371) (V2LY-0425-LY1255)


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Summary

Host Animal
Mouse
Specificity
Mouse, Rat
Clone
XB0371
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 233-272 of mouse E2F-6.
Host Species
Mouse
Specificity
Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
E2F Transcription Factor 6
Entrez Gene ID
Rat313978
Mouse50496
UniProt ID
RatD4ACR8
MouseO54917
Research Area
Inhibitor of E2F-dependent transcription (PubMed:9689056, PubMed:9704927, PubMed:9501179).

Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' (PubMed:9501179).

Has a preference for the 5'-TTTCCCGC-3' E2F recognition site (PubMed:9501179).

E2F6 lacks the transcriptional activation and pocket protein binding domains (PubMed:9704927, PubMed:9501179).

Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression (PubMed:9689056, PubMed:9501179).

Represses expression of some meiosis-specific genes, including SLC25A31/ANT4 (By similarity).

May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase (PubMed:9501179).
Biological Process
Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Ensembl
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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