Mouse Anti-E4F1 Recombinant Antibody (CBFYE-0383) (V2LY-0425-LY1260)

Name: Mouse Anti-E4F1 Recombinant Antibody (CBFYE-0383)
SKU: V2LY-0425-LY1260
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0383

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-240 mapping at the N-terminus of human E4F1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

E4F1

Entrez Gene ID

1877

UniProt ID

Q66K89

Research Area

May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription.

Identified as a cellular target of the adenoviral oncoprotein E1A, it is required for both transcriptional activation and repression of viral genes.

Biological Process

Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
DNA replication Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: GO_Central
Protein ubiquitination Source: UniProtKB-UniPathway
Regulation of cell cycle Source: UniProtKB
Regulation of cell cycle process Source: UniProtKB
Regulation of growth Source: UniProtKB-KW
Regulation of mitotic cell cycle, embryonic Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleoplasm; Cytoplasm. A small fraction is detected in the cytoplasm. Excluded from the nucleolus where it is targeted upon CDKN2A overexpression. Localizes to the mitotic spindle during embryogenesis.

PTM

Proteolytic cleavage produces a 50 kDa N-terminal peptide (p50E4F) which has a DNA-binding activity and activates transcription in presence of the adenoviral E1A protein. The major full-length protein (p120E4F) functions as a repressor of transcription.
Phosphorylated; p120E4F and p50E4F are both phosphorylated. Phosphorylation is cell cycle-dependent and differentially regulates DNA-binding activity and function of both forms.
May be sumoylated by UBE2I upon interaction with CDKN2A.

Batnini, K., Houles, T., Kirsh, O., Du Manoir, S., Zaroual, M., Delpech, H., ... & Rodier, G. (2022). Multi-Level Control of the ATM/ATR-CHK1 Axis by the Transcription Factor E4F1 in Triple-Negative Breast Cancer. International journal of molecular sciences, 23(16), 9217.

Moison, C., Chagraoui, J., Caron, M. C., Gagné, J. P., Coulombe, Y., Poirier, G. G., ... & Sauvageau, G. (2021). Zinc finger protein E4F1 cooperates with PARP-1 and BRG1 to promote DNA double-strand break repair. Proceedings of the National Academy of Sciences, 118(11), e2019408118.

Yan, R. G., Yang, Q. L., & Yang, Q. E. (2020). E4 transcription factor 1 (E4F1) regulates sertoli cell proliferation and fertility in Mice. Animals, 10(9), 1691.

Rooney, R. J. (2020). Multiple domains in the 50 kDa form of E4F1 regulate promoter-specific repression and E1A trans-activation. Gene, 754, 144882.

Zhang, Y., Wan, N., Pan, T., Hu, X., Liu, Q., & Li, S. (2019). MicroRNA-33-3p regulates vein endothelial cell apoptosis in selenium-deficient broilers by targeting E4F1. Oxidative medicine and cellular longevity, 2019.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

E4F1 Matched Antibody Pair (358) (CAT#: APMAB-358LY)

E4F1 Matched Antibody Pair (359) (CAT#: APMAB-359LY)

E4F1 Matched Antibody Pair (360) (CAT#: APMAB-360LY)

Related Products

E4F1 Matched Antibody Pair (359) (CAT#: APMAB-359LY)

Mouse Anti-E4F1 Recombinant Antibody (CBFYE-0384) (CAT#: CBMAB-E0690-FY)

E4F1 Matched Antibody Pair (360) (CAT#: APMAB-360LY)

E4F1 Matched Antibody Pair (358) (CAT#: APMAB-358LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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