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Mouse Anti-E4F1 Recombinant Antibody (CBFYE-0384) (CBMAB-E0690-FY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYE-0384
Antibody Isotype
IgG2a, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
E4F1 (aa685-784) partial recombinant protein.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 685-784

Target

Full Name
E4F1
Introduction
The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence.
Entrez Gene ID
1877
UniProt ID
Q66K89
Alternative Names
E4F
Research Area
May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription.

Identified as a cellular target of the adenoviral oncoprotein E1A, it is required for both transcriptional activation and repression of viral genes.
Biological Process
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
DNA replication Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: GO_Central
Protein ubiquitination Source: UniProtKB-UniPathway
Regulation of cell cycle Source: UniProtKB
Regulation of cell cycle process Source: UniProtKB
Regulation of growth Source: UniProtKB-KW
Regulation of mitotic cell cycle, embryonic Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleoplasm; Cytoplasm. A small fraction is detected in the cytoplasm. Excluded from the nucleolus where it is targeted upon CDKN2A overexpression. Localizes to the mitotic spindle during embryogenesis.
PTM
Proteolytic cleavage produces a 50 kDa N-terminal peptide (p50E4F) which has a DNA-binding activity and activates transcription in presence of the adenoviral E1A protein. The major full-length protein (p120E4F) functions as a repressor of transcription.
Phosphorylated; p120E4F and p50E4F are both phosphorylated. Phosphorylation is cell cycle-dependent and differentially regulates DNA-binding activity and function of both forms.
May be sumoylated by UBE2I upon interaction with CDKN2A.
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For research use only. Not intended for any clinical use.

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