Sign in or Register   Sign in or Register
  |  

Mouse Anti-ECD Antibody (1D10) (CBMAB-0331-YC)

Provided herein are mouse monoclonal antibodies against Human ECD. The antibody clone 1D10 can be used for immunoassay techniques, such as ELISA and IP.
See all ECD antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1D10
Antibody Isotype
IgG2b
Application
ELISA, IP

Basic Information

Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG2b
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ECD
Introduction
ECD (ecdysoneless homolog) belongs to the SGT1 family that is highly expressed in muscle and heart. ECD is a novel promoter of mammalian cell cycle progression. ECD may function to remove the repressive effects of Rb-family tumor suppressors on E2F transcription factors and also is a novel tumor-promoting factor that is differentially expressed in pancreatic cancer and potentially regulates glucose metabolism within cancer cells.
Entrez Gene ID
11319
UniProt ID
O95905
Alternative Names
GCR2; hSGT1
Research Area
Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345).

May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932).

Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839).

The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270).

May play a role in regulation of pre-mRNA splicing (PubMed:24722212).
Biological Process
Cell population proliferation Source: Ensembl
mRNA processing Source: UniProtKB-KW
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of G1/S transition of mitotic cell cycle Source: Ensembl
RNA splicing Source: UniProtKB-KW
Cellular Location
Nucleus; Cytoplasm. Predominantly is located in the cytoplasm.
PTM
Phosphorylated predominantly by CK2 on two serine-containing clusters; involved in cell cycle regulation activity.

Mirza, S., Kalluchi, A., Raza, M., Saleem, I., Mohapatra, B., Pal, D., ... & Band, V. (2022). Ecdysoneless Protein Regulates Viral and Cellular mRNA Splicing to Promote Cervical Oncogenesis. Molecular Cancer Research, 20(2), 305-318.

Holding, N., Raza, M., Band, V., Mohapatra, B. C., & Band, H. (2022). Novel Interaction Between ECD and EIF4A1 Indicates ECD Regulates Eukaryotic Translation.

Saleem, I., Mirza, S., Sarkar, A., Raza, M., Mohapatra, B., Mushtaq, I., ... & Band, V. (2021). The mammalian ecdysoneless protein interacts with RNA helicase DDX39A to regulate nuclear mRNA export. Molecular and cellular biology, 41(7), e00103-21.

Erkelenz, S., Stanković, D., Mundorf, J., Bresser, T., Claudius, A. K., Boehm, V., ... & Uhlirova, M. (2021). Ecd promotes U5 snRNP maturation and Prp8 stability. Nucleic acids research, 49(3), 1688-1707.

Sarkar, A. (2019). Ecdysoneless, a novel regulator of Ca2+ homeostasis and metabolism.

Sarkar, A., Saleem, I., Mirza, S., Bidasee, K., Rao, R., Band, H., & Band, V. (2018). The novel role of ecdysoneless (ECD) in maintain ER Ca2+ homeostasis. Cancer Research, 78(13_Supplement), 448-448.

Saleem, I., Mirza, S., Sarkar, A., Band, H., & Band, V. (2018). Ecdysoneless, a novel regulator of cell cycle progression and oncogenesis. Cancer Research, 78(13_Supplement), 4454-4454.

Xu, S. H., Zhu, S., Wang, Y., Huang, J. Z., Chen, M., Wu, Q. X., ... & Yan, G. R. (2018). ECD promotes gastric cancer metastasis by blocking E3 ligase ZFP91-mediated hnRNP F ubiquitination and degradation. Cell death & disease, 9(5), 1-11.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ECD Antibody (1D10)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare