Mouse Anti-ECD Monoclonal Antibody (1D10) (CBMAB-0331-YC)

Name: Mouse Anti-ECD Monoclonal Antibody (1D10)
SKU: CBMAB-0331-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1D10

Application

ELISA, IP

Immunogen

Recombinant full length of protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	0.2-0.5 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ECD

Introduction

ECD (ecdysoneless homolog) belongs to the SGT1 family that is highly expressed in muscle and heart. ECD is a novel promoter of mammalian cell cycle progression. ECD may function to remove the repressive effects of Rb-family tumor suppressors on E2F transcription factors and also is a novel tumor-promoting factor that is differentially expressed in pancreatic cancer and potentially regulates glucose metabolism within cancer cells.

Entrez Gene ID

11319

UniProt ID

O95905

Alternative Names

GCR2; hSGT1

Research Area

Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345).

May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932).

Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839).

The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270).

May play a role in regulation of pre-mRNA splicing (PubMed:24722212).

Biological Process

Cell population proliferation Source: Ensembl
mRNA processing Source: UniProtKB-KW
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of G1/S transition of mitotic cell cycle Source: Ensembl
RNA splicing Source: UniProtKB-KW

Cellular Location

Nucleus; Cytoplasm. Predominantly is located in the cytoplasm.

PTM

Phosphorylated predominantly by CK2 on two serine-containing clusters; involved in cell cycle regulation activity.

Mirza, S., Kalluchi, A., Raza, M., Saleem, I., Mohapatra, B., Pal, D., ... & Band, V. (2022). Ecdysoneless Protein Regulates Viral and Cellular mRNA Splicing to Promote Cervical Oncogenesis. Molecular Cancer Research, 20(2), 305-318.

Holding, N., Raza, M., Band, V., Mohapatra, B. C., & Band, H. (2022). Novel Interaction Between ECD and EIF4A1 Indicates ECD Regulates Eukaryotic Translation.

Saleem, I., Mirza, S., Sarkar, A., Raza, M., Mohapatra, B., Mushtaq, I., ... & Band, V. (2021). The mammalian ecdysoneless protein interacts with RNA helicase DDX39A to regulate nuclear mRNA export. Molecular and cellular biology, 41(7), e00103-21.

Erkelenz, S., Stanković, D., Mundorf, J., Bresser, T., Claudius, A. K., Boehm, V., ... & Uhlirova, M. (2021). Ecd promotes U5 snRNP maturation and Prp8 stability. Nucleic acids research, 49(3), 1688-1707.

Sarkar, A. (2019). Ecdysoneless, a novel regulator of Ca2+ homeostasis and metabolism.

Sarkar, A., Saleem, I., Mirza, S., Bidasee, K., Rao, R., Band, H., & Band, V. (2018). The novel role of ecdysoneless (ECD) in maintain ER Ca2+ homeostasis. Cancer Research, 78(13_Supplement), 448-448.

Saleem, I., Mirza, S., Sarkar, A., Band, H., & Band, V. (2018). Ecdysoneless, a novel regulator of cell cycle progression and oncogenesis. Cancer Research, 78(13_Supplement), 4454-4454.

Xu, S. H., Zhu, S., Wang, Y., Huang, J. Z., Chen, M., Wu, Q. X., ... & Yan, G. R. (2018). ECD promotes gastric cancer metastasis by blocking E3 ligase ZFP91-mediated hnRNP F ubiquitination and degradation. Cell death & disease, 9(5), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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